A kinetic microplate method for quantifying the antibacterial properties of biological fluids
A kinetic microplate method for quantifying the antibacterial properties of biological fluids
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DOI：
10.1016/s0167-7012(03)00190-8
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发表时间：
2003-11-01
影响因子：
2.2
通讯作者：
Buddington, RK
中科院分区：
文献类型：
--
作者：
Holowachuk, SA;Bal'a, MF;Buddington, RK
测定抑菌活性的微孔板试验采用了已知的庆大霉素浓度范围作为内标。金黄色葡萄球菌亚种以金黄色葡萄球菌ATCC 6538P、大肠杆菌ATCC 25922和乳杆菌为靶菌，也可检测其他指示菌和抗生素。测定范围和灵敏度取决于指示生物的种类和密度，以及条件（例如，生长培养基的类型、浓度和pH值）。绘制生长曲线（AUGC）下的面积与庆大霉素浓度（对数变换）的关系，得到一条线性曲线，用于量化以庆大霉素为单位的分泌液（SCF；胰液）的抗菌活性，并用于比较不同时间、不同日期和不同研究中收集的样品。微滴肉汤法的改进将有助于研究人造化合物、分泌液的抗菌活性以及年龄、饮食和健康状况的影响。(C) 2003 Elsevier B.V.版权所有
The microplate assay for measuring antibacterial activity was adapted by incorporating a known concentration range of gentamicin as an internal standard. Staphylococcus aureus subsp. aureus strain ATCC 6538P, Escherichia coli ATCC 25922, and Lactobacillus spp. were used as target organisms, although other indicator organisms and antibiotics can be examined. Assay range and sensitivity were dependent on the species and density of indicator organism, and conditions (e.g., type, concentration, and pH of growth medium). Plotting the area under the growth curve (AUGC) versus gentamicin concentration (log transformed) yielded a linear curve that was used to quantify in units of gentamicin the antibacterial activity of a secretory fluid (SCF; pancreatic juice) and for comparisons of samples collected at different times, analysed on different days, and from different studies. This adaptation of the microtiter broth method will be useful for investigating man-made compounds, and the antibacterial activity of secretory fluids and the influences of age, diet, and health status. (C) 2003 Elsevier B.V. All rights reserved.