Interaction of progesterone with all or isolated portions of the amphibian (Rana pipiens) oocyte surface. Physical and biological characteristics.
Interaction of progesterone with all or isolated portions of the amphibian (Rana pipiens) oocyte surface. Physical and biological characteristics.
复制标题
黄体酮与两栖动物（Rana pipiens）卵母细胞表面的全部或隔离部分的相互作用。
DOI：
10.1016/0012-1606(77)90134-8
复制
发表时间：
1977
影响因子：
2.7
通讯作者：
A. Schuetz
中科院分区：
文献类型：
--
作者：
J. Cloud;A. Schuetz
黄体酮诱导豚鼠卵母细胞在体内和体外恢复减数分裂成熟。黄体酮与卵母细胞相互作用的性质是用一种技术来研究的，这种技术允许在卵母细胞表面的一部分应用类固醇。[3H]孕酮的摄取随时间和类固醇浓度的变化大致与暴露的表面积成正比。部分卵母细胞表面连续暴露于[3H]孕酮1.5或24小时后，超过90%的放射性与暴露的半球有关。限制卵母细胞表面暴露的部分降低了黄体酮在诱导成熟方面的生物效力，这是通过生殖囊泡破裂来评估的。激素有效性的下降不是由于该技术的直接物理影响。去除表面限制导致类固醇的生物活性增加；类固醇效力的变化与细胞内类固醇分布的增加有关。卵母细胞连续暴露于高水平黄体酮（10 μg/ml）中，在有限的范围内成熟。孵育24小时后，暴露于10 μg/ml孕酮的动物极点上的卵母细胞成熟率为55%，而暴露于植物极点上的卵母细胞成熟率为0%。使用[3H]孕酮，在两个极区摄取或保留的类固醇量没有发现差异。这些研究表明，诱导成熟所需的黄体酮的量与其在卵母细胞中的分布有关，并且动物和植物半球对黄体酮的反应能力不同。
Progesterone induces the resumption of meiotic maturation of fully grown oocytes ofRana pipiensbothin vivoandin vitro. The nature of the interaction of progesterone with the oocyte was investigated using a technique which allowed the application of steroid to a portion of the oocyte surface. Uptake of [3H]progesterone from the incubation media with time and with varying concentrations of steroid was approximately proportional to the surface area exposed. After 1.5 or 24 hr of continuous exposure of a portion of the oocyte surface to [3H]progesterone, greater than 90% of the radioactivity was associated with the hemisphere exposed. Restriction of the portion of oocyte surface exposed reduced the biological potency of progesterone in the induction of maturation as assessed by germinal vesicle breakdown. Decrease in hormone effectiveness was not due to direct physical effects of the technique. Removal of the surface restriction resulted in an increase in biological activity of the steroid; this change in steroid potency was correlated with an increase in steroid distribution over the cell. Oocytes continuously exposed over a restricted part of their surface to high levels of progesterone (10 μg/ml) matured to a limited extent. After 24 hr of incubation, 55% of the oocytes exposed to 10 μg/ml of progesterone over the animal pole matured as compared to 0% of those oocytes exposed over the vegetal pole. Using [3H]progesterone, no difference was detected in the amount of steroid taken up or retained by the two polar regions. These investigations suggest that the amount of progesterone required to induce maturation is related to its distribution over the oocyte and that the animal and vegetal hemispheres differ in their ability to respond to progesterone.