Human prostate-specific glandular kallikrein is expressed as an active and an inactive protein.
Human prostate-specific glandular kallikrein is expressed as an active and an inactive protein.
复制标题
人前列腺特异性腺激肽释放酶表达为活性和非活性蛋白质。
作者：
A. Herrala;R. Kurkela;K. Porvari;R. Isomäki;P. Henttu;P. Vihko
通过对从前列腺癌组织、良性前列腺增生组织和血液白细胞标本中分离的基因组dna进行直接测序（PCR），描述了人类前列腺特异性腺激肽素（hKLK2）基因的多态性。结果显示了两种形式的人类前列腺特异性腺体激肽肽蛋白（hK2），这是hK2编码区碱基792从C到T变化的结果。在昆虫细胞中产生这两种形式的重组蛋白表明Arg226-hK2 （CC基因型）是一种活性蛋白，而Trp226-hK2 （TT基因型）是一种无活性蛋白。36例前列腺疾病患者的多态性研究发现只有1例具有TT基因型。在人前列腺特异性抗原（hKLK3）基因中未发现相同的多态性。Arg226-hK2仅具有胰蛋白酶样酶活性，而重组人前列腺特异性抗原（hPSA）仅具有凝乳胰蛋白酶样酶活性。从精浆中纯化的抗hPSA单克隆和多克隆抗体均可检测活性和失活的hK2。因此，由于不活跃的和稳定的hK2蛋白可能存在，在hPSA标准中缺乏胰蛋白酶样活性不足以确认材料没有hK2污染。
A polymorphism in the human prostate-specific glandular kallikrein (hKLK2) gene was described by direct sequencing (by PCR) of genomic DNAs isolated from prostatic cancer tissue, benign prostatic hyperplasia tissue, and blood leukocyte specimens. Results showed two forms of human prostate-specific glandular kallikrein protein (hK2), a consequence of a change from C to T at base 792 in the hK2 coding region. Producing the two forms as recombinant proteins in insect cells demonstrated that Arg226-hK2 (CC genotype) is an active protein and Trp226-hK2 (TT genotype) is inactive. Polymorphism studies of 36 patients with prostatic diseases identified only 1 with the TT genotype. The same kind of polymorphism was not detected in the human prostate-specific antigen (hKLK3) gene. Arg226-hK2 possessed only trypsin-like enzyme activity, whereas recombinant human prostate-specific antigen (hPSA) had only chymotrypsin-like activity. Monoclonal and polyclonal antibodies raised against hPSA purified from seminal plasma detected both active and inactive hK2. Thus, because inactive as well as stable hK2 protein may be present, a lack of trypsin-like activity in hPSA standards is not enough to confirm that the materials are free of hK2 contamination.
影响因子：
2.9
作者：
YOUNG, CYF;ANDREWS, PE;TINDALL, DJ
通讯作者：
TINDALL, DJ