TRANSCRIPTIONAL REGULATION OF GENE-EXPRESSION IN TETRAHYMENA-THERMOPHILA
TRANSCRIPTIONAL REGULATION OF GENE-EXPRESSION IN TETRAHYMENA-THERMOPHILA
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DOI：
10.1093/nar/18.22.6637
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发表时间：
1990-11-25
影响因子：
14.9
通讯作者：
GOROVSKY, MA
中科院分区：
文献类型：
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作者：
STARGELL, LA;KARRER, KM;GOROVSKY, MA
唯一一项对嗜热四膜虫（Tetrahymena thermophila）基因表达的充分表征研究(1)表明，Ser H3基因的温度依赖性表达在mRNA稳定性水平上受到调控。开发了一种运行转录试验，以确定RNA稳定性的调节是否是四膜虫基因表达的主要机制，或者转录调节是否占主导地位。测定了14个四膜虫基因在不同生理/发育状态（生长、饥饿和偶联）下的相对转录活性，其中许多基因的RNA丰度存在显著差异。除Ser H3外，在所有情况下，转录的变化都伴随着RNA丰度的变化。因此，在四膜虫中，调节RNA丰度的主要机制是差异转录，而不是差异RNA降解。
The only well-characterized study of gene expression in Tetrahymena thermophila (1) demonstrates that the temperature dependent expression of the Ser H3 gene is regulated at the level of mRNA stability. A run-on transcription assay was developed to determine if regulation of RNA stability was a major mechanism regulating gene expression in Tetrahymena or if transcriptional regulation dominates. The relative transcriptional activities of 14 Tetrahymena genes were determined in different physiological/developmental states (growing, starved and conjugating) in which many of the genes showed striking differences in RNA abundance. In every case except Ser H3, changes in transcription accompanied changes in RNA abundance. Thus differential transcription, not differential RNA degradation, is the major mechanism regulating RNA abundance in Tetrahymena.