Effects of simultaneous over-expression of Cu/ZnSOD and MnSOD on Drosophila melanogaster life span
Effects of simultaneous over-expression of Cu/ZnSOD and MnSOD on Drosophila melanogaster life span
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DOI：
10.1016/j.mad.2004.01.009
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发表时间：
2004-05-01
影响因子：
5.3
通讯作者：
Tower, J
中科院分区：
文献类型：
--
作者：
Sun, JT;Molitor, J;Tower, J
基于酵母FLP重组酶的FLP输出技术允许转基因在果蝇成虫中诱导过表达。在FLP缺失的情况下，对照和过表达的果蝇具有相同的遗传背景，因此寿命的差异一定是由转基因诱导引起的。实现的过表达的量在独立的转基因株系之间变化，并且先前对于Cu/ZnSOD和MnSOD的寿命被发现与酶活性的增加成比例地增加。为了确定酶和寿命的更大增加是否可以用FLP-出实现，在含有两个MnSOD转基因的八个品系、含有三个MnSOD转基因的三个品系和含有MnSOD转基因加Cu/ZnSOD转基因的三个品系中分析酶过表达和寿命。寿命再次被发现增加成比例的MnSOD酶活性的增加，平均和最大寿命的增加高达40%。然而，当同时存在多于一个转基因时，每个转基因赋予的酶活性和寿命的增加减少。当考虑到每个转基因的酶的过度表达的效率降低，同时过度表达的MnSOD和Cu/ZnSOD被发现有部分加性效应的寿命。(C)2004爱思唯尔爱尔兰有限公司保留所有权利。
The FLP-out technique, based on yeast FLP recombinase, allows induced over-expression of transgenes in Drosophila adults. With FLP-out control and over-expressing flies have identical genetic backgrounds and therefore differences in life span must result from transgene induction. The amount of over-expression achieved varies between independent transgenic lines, and previously for both Cu/ZnSOD and MnSOD life span was found to be increased in proportion to the increase in enzyme activity. To determine if greater increases in enzyme and life span could be achieved with FLP-out, enzyme over-expression and life span were analyzed in eight lines containing two MnSOD transgenes, three lines containing three MnSOD transgenes, and three lines containing a MnSOD transgene plus a Cu/ZnSOD transgene. Life span was again found to be increased in proportion to the increase in MnSOD enzyme activity, with increases of up to 40% in mean and maximum life span. However the increases in enzyme activity and life span conferred per transgene were reduced when more than one transgene was present at the same time. When the reduced efficiency of enzyme over-expression per transgene was taken into account, simultaneous over-expression of MnSOD and Cu/ZnSOD was found to have partially additive effects on life span. (C) 2004 Elsevier Ireland Ltd. All rights reserved.