AN ULTRASTRUCTURAL-STUDY OF LIPOPROTEIN ACCUMULATION IN CARDIAC VALVES OF THE RABBIT
AN ULTRASTRUCTURAL-STUDY OF LIPOPROTEIN ACCUMULATION IN CARDIAC VALVES OF THE RABBIT
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DOI：
10.1161/01.atv.14.7.1151
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发表时间：
1994-07-01
期刊：
影响因子：
--
通讯作者：
FRANK, J
中科院分区：
文献类型：
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作者：
NIEVELSTEINPOST, P;MOTTINO, G;FRANK, J
心脏瓣膜主要由被内皮细胞单层包围的细胞外基质组成，因此是一个很好的内膜模型。喂食高胆固醇食物的兔子的心脏瓣膜在基质中积累脂质，随着时间的推移，形成与主动脉相似的脂肪条纹。在这项研究中，我们证明了心脏瓣膜（房室和主动脉）可以被分离并用作体外制剂来控制和跟踪低密度脂蛋白（LDL）的摄取和沉积。使用薄切片和冷冻刻蚀显微镜，我们发现LDL与细胞外基质内的胶原蛋白迅速结合。当它沿着胶原纤维积累时，LDL在大小和表面形貌上发生结构变化。低密度脂蛋白与胶原的这种关联可能是内膜脂质聚集的关键步骤。
Heart valves are composed chiefly of extracellular matrix surrounded by an endothelial cell monolayer and as a result are an excellent model of the intima. Heart valves from rabbits fed a high-cholesterol diet accumulate lipids within the matrix and over time develop fatty streaks similar to those seen in the aorta. In this study we demonstrate that the heart valves (atrioventricular and aortic) can be isolated and used as an in vitro preparation to control and follow low-density lipoprotein (LDL) uptake and deposition. Using thin-section and freeze-etch microscopy we found that LDL rapidly associates with collagen within the extracellular matrix. As it accumulates along the collagen fibers the LDL appears to undergo structural changes in size and surface topography. This association of LDL with collagen may be a key step in lipid aggregation in the intima.