The proteome of the infectious bronchitis virus Beau-R virion
The proteome of the infectious bronchitis virus Beau-R virion
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DOI：
10.1099/jgv.0.000304
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发表时间：
2015-12-01
影响因子：
3.8
通讯作者：
Maier, Helena J.
中科院分区：
文献类型：
--
作者：
Dent, Stuart D.;Xia, Dong;Maier, Helena J.
传染性支气管炎是由冠状病毒传染性支气管炎病毒（IBV）引起的禽类高度接触性呼吸道疾病。人们认为冠状病毒病毒体由三种主要的病毒结构蛋白组成，直到对其他冠状病毒的研究表明病毒体还包括病毒非结构蛋白和属特异性辅助蛋白以及宿主细胞蛋白。为了研究IBV病毒体的蛋白质组，病毒在鸡胚中生长，通过蔗糖梯度离心纯化并通过质谱分析。分析纯化的IBV的三种制备物产生了三种预期的结构蛋白加上35种额外的病毒体相关宿主蛋白。病毒体相关宿主蛋白具有多种功能属性，参与细胞骨架形成、RNA结合和蛋白质折叠途径。其中一些蛋白质是本研究所独有的，而另一些蛋白质则被发现与严重急性呼吸综合征冠状病毒粒子以及许多其他RNA和DNA病毒粒子中鉴定的蛋白质是同源的。
Infectious bronchitis is a highly contagious respiratory disease of poultry caused by the coronavirus infectious bronchitis virus (IBV). It was thought that coronavirus virions were composed of three major viral structural proteins until investigations of other coronaviruses showed that the virions also include viral non-structural and genus-specific accessory proteins as well as host-cell proteins. To study the proteome of IBV virions, virus was grown in embryonated chicken eggs, purified by sucrose-gradient ultracentrifugation and analysed by mass spectrometry. Analysis of three preparations of purified IBV yielded the three expected structural proteins plus 35 additional virion-associated host proteins. The virion-associated host proteins had a diverse range of functional attributions, being involved in cytoskeleton formation, RNA binding and protein folding pathways. Some of these proteins were unique to this study, while others were found to be orthologous to proteins identified in severe acute respiratory syndrome coronavirus virions and also virions from a number of other RNA and DNA viruses.