Paranemic cohesion of topologically-closed DNA molecules
Paranemic cohesion of topologically-closed DNA molecules
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DOI：
10.1021/ja026973b
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发表时间：
2002-11-06
影响因子：
15
通讯作者：
Seeman, NC
中科院分区：
文献类型：
--
作者：
Zhang, XP;Yan, H;Seeman, NC
DNA分子的特定凝聚力是生物技术、DNA纳米技术和基于DNA的计算工作成功的关键。双螺旋之间最常见的分子间结合形式是通过粘性末端，但是由天然存在的限制性酶产生的粘性末端通常可能太短而不能将大的构建体结合在一起。另一种结合形式是通过平行交叉（PX）基序。PX基序的两个组分中的每一个都可以是DNA哑铃或其他拓扑封闭的种类。哑铃的交替半圈在分子内配对。中间的半匝与相对分量的半匝成对。我们证明了PX凝聚力的功效，表明它可以导致两个三角形基序，每个含有近500个核苷酸的1：1的结合。内聚完成，证明了通过粘性末端结合核酸分子的替代方案。
Specific cohesion of DNA molecules is key to the success of work in biotechnology, DNA nanotechnology and DNA-based computation. The most common form of intermolecular cohesion between double helices is by sticky ends, but sticky ends generated by naturally occurring restriction enzymes may often be too short to bind large constructs together. An alternative form of binding is available through the paranemic crossover (PX) motif. Each of the two components of a PX motif can be a DNA dumbbell or other topologically closed species. Alternate half-turns of the dumbbell are paired intramolecularly. The intervening half-turns are paired with those of the opposite component. We demonstrate the efficacy of PX cohesion by showing that it can result in the 1:1 binding of two triangle motifs, each containing nearly 500 nucleotides. The cohesion goes to completion, demonstrating an alternative to binding nucleic acid molecules through sticky ends.