Hypoglycemic effects of Tibetan medicine Huidouba in STZ-induced diabetic mice and db/db mice.
Hypoglycemic effects of Tibetan medicine Huidouba in STZ-induced diabetic mice and db/db mice.
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藏药灰豆巴对STZ诱导的糖尿病小鼠和db/db小鼠的降血糖作用
DOI：
10.1016/j.chmed.2021.02.001
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发表时间：
2021-04
影响因子：
3.8
通讯作者：
中科院分区：
文献类型：
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作者：
灰豆霸（HDB）是中国四川省用于治疗糖尿病的中药。因此，我们研究了 HDB 的抗糖尿病作用及其潜在机制。我们假设 HDB 治疗可以增强葡萄糖耐量和胰岛素敏感性，从而预防高血糖状态。为了检验这一假设，链脲佐菌素 (STZ) 诱导的糖尿病小鼠和 db/db 小鼠（广泛使用的高血糖和胰岛素抵抗糖尿病模型）接受 HDB、二甲双胍或阿卡波糖治疗。检测血糖、口服葡萄糖耐量试验、胰岛素耐量试验、胰腺组织病理学和血清生化，评估HDB的降血糖作用。研究发现 HDB 治疗具有降低血糖水平的效果。 HDB 还导致 STZ 诱导的糖尿病小鼠模型的体重和食物摄入量显着减少。此外，它还显着改善了两种糖尿病小鼠模型的葡萄糖和胰岛素耐受性。重要的是，胰岛素、胰高血糖素、胰多肽和生长抑素免疫组织化学显示，与其他两种治疗相比，HDB 治疗改善了胰岛细胞的功能和位置。 HDB 治疗导致胰岛功能显着恢复。我们的结果阐明了 HDB 在糖尿病进展中的潜在机制，HDB 可以成为治疗糖尿病的有效药物。这项研究的结果表明，HDB 可以降低 STZ 诱导的高血糖小鼠和 db/db 小鼠的血糖水平。
Huidouba (HDB) is a Chinese folk medicine used to treat diabetes in Sichuan Province, China. Therefore, we investigated the anti-diabetic effects of HDB and its underlying mechanisms. We hypothesized that HDB treatment could enhance glucose tolerance and insulin sensitivity, and thus prevent a hyperglycemia state. To test the hypothesis, streptozotocin (STZ)-induced diabetic mice and db/db mice, widely used models of hyperglycemia and insulin-resistant diabetes, were either treated with HDB, metformin, or acarbose. Blood glucose, oral glucose tolerance test, insulin tolerance test, pancreatic histopathology and serum biochemistry were detected to assess the hypoglycemic effect of HDB. HDB treatments were found to show the effect in reducing glucose levels. HDB also resulted in a significant reduction in body weight and food intake in the STZ-induced diabetic mouse model. Furthermore, it significantly improved glucose and insulin tolerance in the two diabetic mouse models. Importantly, insulin, glucagon, pancreatic polypeptide, and somatostatin immunohistochemistry revealed that HDB treatment improved the function and the location of the cells in the islets compared with the other two treatments. HDB treatment resulted in significant restoration of islet function. Our results illustrated the underlying mechanism of HDB in the progression of diabetes, and HDB can be an effective agent for the treatment of diabetes. The results of this study suggested that HDB can reduce blood glucose levels in STZ-induced hyperglycemic mice and db/db mice.