Development of a molecular marker for the adult plant leaf rust resistance gene Lr35 in wheat
Development of a molecular marker for the adult plant leaf rust resistance gene Lr35 in wheat
复制标题
DOI：
10.1007/s001220051268
复制
发表时间：
1999-08-01
影响因子：
5.4
通讯作者：
Keller, B
中科院分区：
文献类型：
--
作者：
Seyfarth, R;Feuillet, C;Keller, B
本工作的目的是开发一个成年植物叶锈病抗性基因Lr35的标记。Lr35基因最初渗入染色体2B中。斯卑尔脱小麦是小麦的一个二倍体近缘种。分析了96个来自抗性系ThatcherLr 35和感病品种Frisal之间杂交的F-2植株的分离群体。在先前定位于小麦2B染色体上的80个RFLP探针中，有51个在杂交亲本之间检测到多态性。其中3个与抗病基因Lr 35完全连锁。将共分离探针BCD 260转化为基于PCR的序列标记位点（STS）标记。一组48个不同的育种系来自几个欧洲育种计划进行了测试与STS标记。这些品系在其系谱中均不具有Lr35的供体，并且它们都与STS标记呈阴性反应。由于在世界各地均未发现对Lr 35有致病力的叶锈病小种，因此Lr 35抗性基因的STS标记对于支持该基因与其它叶锈病基因组合通过标记辅助选择渗入育种材料中具有重要价值。
The objective of this work was to develop a marker for the adult plant leaf rust resistance gene Lr35. The Lr35 gene was originally introgressed into chromosome 2B from. Triticum speltoides, a diploid relative of wheat. A segregating population of 96 F-2 plants derived from a cross between the resistant line ThatcherLr35 and the susceptible variety Frisal was analysed. Out of 80 RFLP probes previously mapped on wheat chromosome 2B, 51 detected a polymorphism between the parents of the cross. Three of them were completely Linked with the resistance gene Lr35. The co-segregating probe BCD260 was converted into a PCR-based sequence-tagged-site (STS) marker. A set of 48 different breeding lines derived from several European breeding programs was tested with the STS marker. None of these lines has a donor for Lr35 in its pedigree and all of them reacted negatively with the STS marker. As no leaf rust races virulent on Lr35 have been found in different areas of the world, the STS marker for the Lr35 resistance gene is of great value to support the introgression of this gene in combination with other leaf rust (Lr) genes into breeding material by marker-assisted selection.