QIAamp MinElute Virus kit effectively extracts viral nucleic acids from cerebrospinal fluids and nasopharyngeal swabs
QIAamp MinElute Virus kit effectively extracts viral nucleic acids from cerebrospinal fluids and nasopharyngeal swabs
复制标题
DOI：
10.1016/j.jcv.2005.05.011
复制
发表时间：
2006-02-01
影响因子：
8.8
通讯作者：
Tang, YW
中科院分区：
文献类型：
--
作者：
Sefers, SE;Rickmyre, J;Tang, YW
背景：从各种临床标本中制备核酸需要有效的靶向回收和扩增抑制物的去除，这是成功的分子诊断的关键。将QIAAMP Minelute病毒试剂盒(Qiagen Inc.，Valencia，CA)与我们实验室目前使用的两种方法进行比较：IsoQuick(Orca Research Inc.，Bothell，WA)用于提取DNA，RNAzol B(Leedo实验室Inc.，Houston，TX)用于提取病毒核酸。研究设计：使用150例临床标本，包括脑脊液(CSF)和鼻咽拭子(NPS)，确定Minelute与其他两种方法的提取效率。结果：MinElute试剂盒与IsoQuick/RNAzol试剂盒检测单纯疱疹病毒(HSV)、EB病毒(EBV)、水痘带状疱疹病毒(VZV)、甲型流感病毒和肠道病毒的结果完全一致。这些试剂盒在检测DNA或RNA方面具有同等的能力，具有更好的恢复高质量和高数量的RNA的能力。MinElute试剂盒具有处理更大样本容量的潜力，可以将处理时间从2小时缩短到50-55分钟。结论：虽然试剂盒成本相对较高，但MinElute试剂盒为诊断分子微生物学实验室提供了另一种快速且用户友好的标本处理工具。(C)2005 Elsevier B.V.保留所有权利。
Background: Nucleic acid preparation from a variety of clinical specimens requires efficient target recovery and amplification inhibitor removal and is critical for successful molecular diagnosis. The QIAamp MinElute Virus kit (Qiagen Inc., Valencia, CA) was compared to the two existing methods currently used in our laboratory, IsoQuick (Orca Research Inc., Bothell, WA) for DNA extraction and RNAzol B (Leedo Laboratories Inc., Houston, TX) for RNA extraction, of viral nucleic acids.Study design: A total of 150 clinical specimens, including cerebrospinal fluid (CSF) and nasopharyngeal swabs (NPS), were used to determine the extraction efficiency of the MinElute compared to the other two methods. Nucleic acid recovery, hands-on time, turn-around-time and cost were compared across all kits.Results: There was complete concordance between the MinElute and IsoQuick/RNAzol kits when herpes simplex virus (HSV), Epstein-Barr virus (EBV), varicella-zoster virus (VZV), influenza A virus or enteroviruses were detected using a colorimetric microliter plate PCR system. The kits were equivalent in their ability to detect either DNA or RNA with Superior ability to recover a high quality and quantity of RNA. With the potential to process larger specimen volumes, the MinElute kit can significantly shorten processing time from 2 h to 50-55 min.Conclusions: Although relatively high test kit costs were noted, the MinElute kit provides another rapid and user-friendly specimen processing tool in the diagnostic molecular microbiology laboratory. (C) 2005 Elsevier B.V. All rights reserved.