Bacterial Diversity in Linglong Gold Mine, China
Bacterial Diversity in Linglong Gold Mine, China
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中国玲珑金矿细菌多样性
DOI：
10.1080/01490451.2016.1186763
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发表时间：
2017-01-01
影响因子：
2.3
通讯作者：
Duan, Jinming
中科院分区：
文献类型：
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作者：
Li, Mengjie;Tian, Haixia;Duan, Jinming
摘要细菌一直在积极地调节环境中各种元素的循环。为探讨细菌在金矿地球化学循环中的作用，采用16S rRNA基因克隆文库分析和培养方法，对玲珑金矿矿区岩石和表层土壤中的细菌多样性进行了分析。在MR中共鉴定出24个操作分类单位（OTUs），涵盖3门18属。同时，从SS中鉴定出24个OTU，包括4门18属。与16S rRNA基因克隆文库分析结果比较，共获得需氧菌28株，厌氧菌34株，而SS中则分别获得需氧菌26株，厌氧菌71株。可培养细菌分属于厚壁菌门、变形菌门和放线菌门，以厚壁菌门为主。这些结果强调了金矿中细菌多样性的高水平。本研究为玲珑金矿区微生物多样性研究提供了新的信息，为揭示细菌在金矿地球化学循环中的存在及其潜在作用提供了新的思路。
ABSTRACT Bacteria have been actively regulating cycles of various elements in the environment. To explore the potential bacterial role in gold biogeochemical cycling, this study analyzed the bacterial diversity of mine rock (MR) and surface soil (SS) samples from Linglong gold mine using 16S rRNA gene clone library analysis and cultivation method. From MR, 24 operational taxonomic units (OTUs) were identified from MR, covering 3 phyla and 18 genera. Meanwhile, 24 OTUs were identified from SS, including 4 phyla and 18 genera. Compared with 16S rRNA gene clone library analysis, 28 aerobic and 34 anaerobic isolates were obtained, whereas 26 aerobic and 71 anaerobic strains were isolated from SS. The cultivable bacteria were affiliated with Firmicutes, Proteobacteria and Actinobacteria phyla, and dominated by Firmicutes. These results underscore the high level of bacterial diversity in the gold mine. Our study provides information on the microbial diversity in Linglong gold mine and sheds light on the existence and potential function of bacteria in the gold biogeochemical cycling.