A Golden Gate Modular Cloning Toolbox for Plants
A Golden Gate Modular Cloning Toolbox for Plants
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DOI：
10.1021/sb4001504
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发表时间：
2014-11-01
影响因子：
4.7
通讯作者：
Marillonnet, Sylvestre
中科院分区：
文献类型：
--
作者：
Engler, Carola;Youles, Mark;Marillonnet, Sylvestre
植物合成生物学需要强大而有效的方法来组装多基因结构。金门克隆技术提供了一种基于精确模块的克隆技术，可以在一个构建体中方便地组装多个基因。我们为植物生物学家提供了一个多功能资源，包括一组克隆载体和96个标准化部分，以实现植物转化的多基因构建的金门构建。部分包括启动子、未翻译序列、报告子、抗原标签、定位信号、可选择标记和终止子。讨论了模型植物烟叶各部件的性能比较。
Plant Synthetic Biology requires robust and efficient methods for assembling multigene constructs. Golden Gate cloning provides a precision module-based cloning technique for facile assembly of multiple genes in one construct. We present here a versatile resource for plant biologists comprising a set of cloning vectors and 96 standardized parts to enable Golden Gate construction of multigene constructs for plant transformation. Parts include promoters, untranslated sequences, reporters, antigenic tags, localization signals, selectable markers, and terminators. The comparative performance of parts in the model plant Nicotiana benthamiana is discussed.