Basic fibroblast growth factor enhances stemness of human stem cells from the apical papilla.
Basic fibroblast growth factor enhances stemness of human stem cells from the apical papilla.
复制标题
碱性成纤维细胞生长因子增强人类根尖乳头干细胞的干细胞性。
DOI：
10.1016/j.joen.2012.01.014
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发表时间：
2012-05
影响因子：
4.2
通讯作者：
Ni L
中科院分区：
文献类型：
--
作者：
Wu J;Huang GT;He W;Wang P;Tong Z;Jia Q;Dong L;Niu Z;Ni L
根尖乳头干细胞（Stem cells from the apical papilla，SCAP）是一种存在于未成熟恒牙根尖乳头发育组织中的间充质干细胞。研究表明，SCAP可能是一个来源的初级成牙本质细胞，负责形成根牙本质。碱性成纤维细胞生长因子（bFGF）是一种信号分子和多效性生长因子，参与牙根发育，并促进多种细胞类型的增殖。然而，bFGF对SCAP的影响尚未得到研究。我们研究了bFGF对体外培养的人SCAP增殖和分化潜能的调节作用。评价了在有或没有bFGF的情况下培养的SCAP的细胞周期和增殖、集落形成单位-成纤维细胞形成、碱性磷酸酶（ALP）活性、成骨/牙本质分化以及干细胞基因标记物的变化。5 ng/mL bFGF处理显著增加SCAP增殖和它们的集落形成单位成纤维细胞形成效率。生长因子还增加了STRO-1和干细胞基因标记Nanog、Oct 4、Sox 2和Rex 1在SCAP中的表达。相反，碱性成纤维细胞生长因子减少ALP活性，矿物质结节的形成，和表达的ALP，骨钙素，骨唾液蛋白，牙本质唾液磷蛋白。当SCAP培养物在bFGF存在下扩增1周时，随后的成骨/牙本质形成条件的刺激导致分化增强。在一定条件下，bFGF通过上调干细胞基因表达、增加增殖能力和增强分化潜能来增强SCAP干性。
Stem cells from the apical papilla (SCAP) are a type of mesenchymal stem cells found in the developing tissue, apical papilla, of immature permanent teeth. Studies have shown that SCAP are likely to be a source of primary odontoblasts that are responsible for the formation of root dentin. Basic fibroblast growth factor (bFGF) is a signaling molecule and pleiotropic growth factor involved in tooth root development, and it promotes proliferation of a variety of cell types. The effects of bFGF on SCAP, however, have not been examined. We investigated the regulatory effects of bFGF on the proliferation and differentiation potential of human SCAP in vitro. Changes in the cell cycle and proliferation, colony-forming unit–fibroblastic formation, alkaline phosphatase (ALP) activity, osteogenic/dentinogenic differentiation, and stem cell gene makers of SCAP, cultured in the presence or absence of bFGF, were evaluated. Treatment with 5 ng/mL bFGF significantly increased SCAP proliferation and their colony-forming unit–fibroblastic formation efficiency. The growth factor also increased the expression of STRO-1 and the stem cell gene makers Nanog, Oct4, Sox2, and Rex1 in SCAP. In contrast, bFGF reduced the ALP activity, mineral nodule formation, and the expression of ALP, osteocalcin, bone sialoprotein, and dentin sialophosphoprotein. When SCAP cultures were expanded in the presence of bFGF for 1 week, subsequent stimulation of the osteogenic/dentinogenic condition resulted in enhanced differentiation. Under certain conditions, bFGF enhances SCAP stemness by up-regulating stem cell gene expression, increasing proliferation ability, and potentiating differentiation potency.