Transcription units of adenovirus type 2. Termination of transcription beyond the poly(A) addition site in early regions 2 and 4.
Transcription units of adenovirus type 2. Termination of transcription beyond the poly(A) addition site in early regions 2 and 4.
复制标题
2 型腺病毒的转录单位。在早期区域 2 和 4 中超出 Poly(A) 添加位点的转录终止。
DOI：
10.1016/0022-2836(80)90096-0
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发表时间：
1980
影响因子：
5.6
通讯作者：
DarnellJr,JE
中科院分区：
文献类型：
--
作者：
Nevins,JR;Blanchard,JM;DarnellJr,JE
对感染周期早期细胞腺病毒2区（~50-75）互补序列的脉冲标记核RNA进行的三种分析表明，转录有规律地通过poly（A）位点进行：（1）新生链在图位61·6处poly（A）位点前后的序列转录摩尔浓度相等;（2）含有poly（A）位点远端序列的新生RNA分子的大小大于poly（A）位点附近的分子的大小;（3）紫外光转录分析表明，poly（A）以外的序列与其它序列相比，位点是似乎起始于2型腺病毒基因组上的图谱位置75处的转录单位的一部分。还确定了早期区域4的转录也在poly（A）添加位点（位于图谱位置91·5）之外进行，可能终止于图谱位置88的区域。
Three types of analysis of pulse-labeled nuclear RNA complementary to adenovirus region 2 (~50–75) from cells early in the infectious cycle showed that transcription regularly proceeded past the poly(A) site: (1) nascent chains showed equal molarity of transcription of sequences before and after the poly(A) site at map position 61·6; (2) the size of the nascent RNA molecules containing the sequences distal to the poly(A) site was larger than the size of the molecules proximal to the poly(A) site; (3) ultraviolet light transcription analysis indicated that the sequences beyond the poly(A) site were part of the leftward transcription unit that appears to initiate at map position 75 on the adenovirus type 2 genome. It was also determined that transcription of early region 4 also proceeds beyond the poly(A) addition site (located at map position 91·5), probably terminating in the region of map position 88.