Ent5p is required with Ent3p and Vps27p for ubiquitin-dependent protein sorting into the multivesicular body
Ent5p is required with Ent3p and Vps27p for ubiquitin-dependent protein sorting into the multivesicular body
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DOI：
10.1091/mbc.e03-11-0793
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发表时间：
2004-07-01
影响因子：
3.3
通讯作者：
Friant, S
中科院分区：
文献类型：
--
作者：
Eugster, A;Pécheur, EI;Friant, S
在晚期内涵体，运往液泡内部的货物被分类到多泡体的内陷囊泡中。 PtdIns(3,5)P 和泛素对于其中一些货物的正确分类都是必需的。我们发现 Ent5p 是一种与 Ent3p 同源的 epsin 家族酵母蛋白，定位于核内体并通过其 ENTH 结构域特异性结合 PtdIns(3,5)P-2。在缺乏 Ent3p 和 Ent5p 的细胞中，生物合成和内吞货物进入多泡体的泛素依赖性分类被破坏，而其他通往液泡的运输途径不受影响。 Ent3p 和 Ent5p 与 Vps27p 相关，Vps27p 是一种包含 FYVE 结构域的蛋白质，可与泛素化货物相互作用，并且是蛋白质分选到多囊泡体中所必需的。因此，Ent3p 和 Ent5p 是第一个被证明是 PtdIns(3,5)P-2(-) 和多囊泡体中 Vps27p 泛素驱动的分选机器之间连接器的蛋白质。
At the late endosomes, cargoes destined for the interior of the vacuole are sorted into invaginating vesicles of the multivesicular body. Both PtdIns(3,5)P, and ubiquitin are necessary for proper sorting of some of these cargoes. We show that Ent5p, a yeast protein of the epsin family homologous to Ent3p, localizes to endosomes and specifically binds to PtdIns(3,5)P-2 via its ENTH domain. In cells lacking Ent3p and Ent5p, ubiquitin-dependent sorting of biosynthetic and endocytic cargo into the multivesicular body is disrupted, whereas other trafficking routes to the vacuole are not affected. Ent3p and Ent5p are associated with Vps27p, a FYVE domain containing protein that interacts with ubiquitinated cargoes and is required for protein sorting into the multivesicular body. Therefore, Ent3p and Ent5p are the first proteins shown to be connectors between PtdIns(3,5)P-2(-) and the Vps27p-ubiquitin-driven sorting machinery at the multivesicular body.