MALDI TIMS IMS of Disialoganglioside Isomers─GD1a and GD1b in Murine Brain Tissue
MALDI TIMS IMS of Disialoganglioside Isomers─GD1a and GD1b in Murine Brain Tissue
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小鼠脑组织中二唾液酸神经节苷脂异构体 GD1a 和 GD1b 的 MALDI TIMS IMS
DOI：
10.1021/acs.analchem.2c03939
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发表时间：
2022
影响因子：
7.4
通讯作者：
Spraggins, Jeffrey M.
中科院分区：
文献类型：
--
作者：
Djambazova, Katerina V.;Dufresne, Martin;Migas, Lukasz G.;Kruse, Angela R.;Van de Plas, Raf;Caprioli, Richard M.;Spraggins, Jeffrey M.
神经节苷脂是一种酸性鞘糖脂，含有神经酰胺部分和具有一个或多个唾液酸残基的寡糖链，并且是具有不同生物学作用的高度多样化的异构体结构。基质辅助激光解吸/电离成像质谱 (MALDI IMS) 可以直接从组织切片中对神经节苷脂和其他生物分子进行非靶向空间分析。将俘获离子淌度光谱法与 MALDI IMS 相结合，可以对异构脂质结构进行原位分析。在这里，我们展示了多个样品中唾液酸残基位置不同的二唾液酸神经节苷脂异构体 GD1a 和 GD1b 的气相分离和鉴定，包括两种异构体的标准混合物、生物提取物和直接来自薄组织切片的样品。在大鼠海马和脊髓组织切片中确定了 GD1a/b (d36:1) 和 GD1a/b (d38:1) 异构体的独特空间分布，证明了基于碳水化合物链和神经酰胺部分对神经节苷脂进行结构表征和空间绘图的能力。
Gangliosides are acidic glycosphingolipids, containing ceramide moieties and oligosaccharide chains with one or more sialic acid residue(s) and are highly diverse isomeric structures with distinct biological roles. Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) enables the untargeted spatial analysis of gangliosides, among other biomolecules, directly from tissue sections. Integrating trapped ion mobility spectrometry with MALDI IMS allows for the analysis of isomeric lipid structures in situ. Here, we demonstrate the gas-phase separation and identification of disialoganglioside isomers GD1a and GD1b that differ in the position of a sialic acid residue, in multiple samples, including a standard mixture of both isomers, a biological extract, and directly from thin tissue sections. The unique spatial distributions of GD1a/b (d36:1) and GD1a/b (d38:1) isomers were determined in rat hippocampus and spinal cord tissue sections, demonstrating the ability to structurally characterize and spatially map gangliosides based on both the carbohydrate chain and ceramide moieties.