Inhibition of glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase in sea urchin eggs by palymitoyl-coenzyme A and reversal by polyamines.
Inhibition of glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase in sea urchin eggs by palymitoyl-coenzyme A and reversal by polyamines.
复制标题
多酰胺酰辅酶 A 对海胆卵中葡萄糖 6-磷酸脱氢酶和 6-磷酸葡萄糖酸脱氢酶的抑制作用以及多胺的逆转作用。
DOI：
10.1016/0003-9861(80)90517-2
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发表时间：
1980
影响因子：
3.9
通讯作者：
I. Yasumasu
中科院分区：
文献类型：
--
作者：
Masatoshi Mita;I. Yasumasu
棕榈酰辅酶A对海胆卵中的粗葡萄糖6-磷酸脱氢酶和6-磷酸葡萄糖酸脱氢酶有抑制作用。使用 0.43 ± 0.05 μmp 棕榈酰辅酶 A 可以抑制未受精卵上清液中的葡萄糖 6-磷酸脱氢酶，使用 4.41 ± 0.20 μmp 棕榈酰辅酶 A 可以抑制 6-磷酸葡萄糖酸脱氢酶 50%。此外，受精卵中的这些酶在受精后 30 分钟受到棕榈酰辅酶 A 的抑制，几乎与未受精卵中的一样。棕榈酸钠、辅酶A、乙酰辅酶A、棕榈酰肉碱和肉碱未能对这些脱氢酶的活性产生任何抑制作用。未受精卵中长链脂肪酰辅酶A的细胞内浓度（3.08±0.33nmol/106个卵）足够高，足以抑制这些酶，并在受精后下降至较低水平（受精后30分钟1.49±0.08nmol/106个卵）。精胺和亚精胺取消了棕榈酰辅酶A对这些酶的抑制作用。鉴于棕榈酰辅酶A对葡萄糖6-磷酸脱氢酶和6-磷酸葡萄糖酸脱氢酶的抑制作用，戊糖单磷酸循环中的这些脱氢酶可能在未受精卵中受到长链脂肪酰辅酶A的抑制，并通过受精后长链酰辅酶A水平的降低和多胺水平的增加而从抑制状态中释放出来。
Palmitoyl-CoA inhibited crude glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase in the eggs of the sea urchin,Hemicentrotus pulcherrimus. Fifty percent inhibition of the glucose 6-phosphate dehydrogenase in the supernatant of unfertilized eggs was obtained with 0.43 ± 0.05 μmpalmitoyl-CoA, and of 6-phosphogluconate dehydrogenase with 4.41 ± 0.20 μmpalmitoyl-CoA. Also, these enzymes in fertilized eggs 30 min after fertilization were inhibited by palmitoyl-CoA almost as much as in unfertilized eggs. Na-Palmitate, coenzyme A, acetyl-CoA, palmitoylcarnitine, and carnitine failed to exert any inhibitory effect on the activities of these dehydrogenases. The intracellular concentration of long-chain fatty acyl-CoA in unfertilized eggs (3.08 ± 0.33 nmol/106eggs) was high enough for the inhibition of these enzymes, and decreased following fertilization to a low level (1.49 ± 0.08 nmol/106eggs 30 min after fertilization). Spermine and spermidine canceled the inhibition of these enzymes by palmitoyl-CoA. In view of the inhibition of glucose 6-phosphate dehydrogenase and of 6-phosphogluconate dehydrogenase by palmitoyl-CoA, these dehydrogenases in the pentose monophosphate cycle are probably inhibited in unfertilized eggs by long-chain fatty acyl-CoA and released from the inhibited state by both the decrease in the level of long-chain acyl-CoA and the increase in the level of polyamines following fertilization.