Expression of membrane prostaglandin E synthase in human placenta and fetal membranes and effect of labor
Expression of membrane prostaglandin E synthase in human placenta and fetal membranes and effect of labor
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DOI：
10.1385/endo:20:3:219
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发表时间：
2003-04-01
期刊：
影响因子：
3.7
通讯作者：
Gibb, W
中科院分区：
文献类型：
--
作者：
Alfaidy, N;Sun, M;Gibb, W
人类分娩的开始和维持与子宫内组织合成前列腺素的增加有关。本研究的目的是研究胎盘、胎膜和蜕膜中膜结合蛋白(MPGES)蛋白和mPGES mRNA在足月胎盘、胎膜和蜕膜中的分布，并确定分娩过程中是否发生任何变化。MPGES在羊膜上皮细胞和绒毛滋养层细胞中高度集中，在间充质层中表达较低。这种酶在蜕膜组织中处于非常低的水平或检测不到。胎盘组织中mPGES蛋白和mRNA的表达水平明显低于胎膜组织。在血管周围的细胞中，mPGES与合体滋养体相关。胎膜和胎盘组织中mPGES基因的表达无明显变化，但Western分析显示，胎盘组织中mPGES蛋白表达下降，绒毛膜组织中mPGES蛋白表达增加，羊膜组织中mPGES蛋白表达无明显变化。胎盘、绒毛膜和羊膜在分娩前后表达的差异表明，这种酶在这些组织中的表达在这个时候是不同的。
Initiation and maintenance of labor in humans is associated with an increase in prostaglandin synthesis by intrauterine tissues. The objective of the present study was to characterize the distribution of membrane-bound PGES (mPGES) protein and mPGES mRNA in human placenta, fetal membranes, and decidua at term and to determine whether any changes occurred with labor. Immunoreactive mPGES was found to be highly concentrated in amnion epithelial cells and the chorion laeve trophoblasts, with lower levels in the mesenchymal layers. The enzyme was at very low levels or undetectable in the decidual tissue. Much lower levels of mPGES protein and mRNA were found in placenta than in fetal membranes. mPGES was associated with the syncytiotrophob last and in cells surrounding blood vessels. The expression of mPGES mRNA did not change with labor in full membranes or placenta, but Western analysis showed an increase in mPGES protein in chorion laeve and a decrease in mPGES protein in placenta during labor, with no change in the amnion. The differences in expression found among placenta, chorion, and amnion before and after labor would indicate that this enzyme is differentially regulated in these tissues at this time.