Cytoskeletal self-organization in neuromorphogenesis.
Cytoskeletal self-organization in neuromorphogenesis.
复制标题
DOI：
10.4161/bioa.29070
复制
发表时间：
2014-03
期刊：
影响因子：
--
通讯作者：
Dehmelt L
中科院分区：
文献类型：
--
作者：
Dehmelt L
动态微管通过与相关电机相互作用的自组织在主轴形成中起着关键作用。细胞形态发生的其他方面，如神经元细胞突起的形成和发展，其基于微管的机制尚不清楚。在最近的一项研究中，我们研究了通过表达神经元微管稳定剂MAP2c在非神经元细胞中诱导微管大规模重组的分子机制。在这项研究中，我们直接观察了皮质动力蛋白复合物以及它们如何影响活细胞中运动微管的动态行为。我们发现，固定动力蛋白复合物与细胞皮层附近的运动微管有短暂的联系，它们的快速周转促进了有效的微管运输。在这里，我们在细胞形态发生的大背景下讨论我们的发现，特别关注自组织原则，细胞形状模式，如神经元的细突起可以出现。
Self-organization of dynamic microtubules via interactions with associated motors plays a critical role in spindle formation. The microtubule-based mechanisms underlying other aspects of cellular morphogenesis, such as the formation and development of protrusions from neuronal cells is less well understood. In a recent study, we investigated the molecular mechanism that underlies the massive reorganization of microtubules induced in non-neuronal cells by expression of the neuronal microtubule stabilizer MAP2c. In that study we directly observed cortical dynein complexes and how they affect the dynamic behavior of motile microtubules in living cells. We found that stationary dynein complexes transiently associate with motile microtubules near the cell cortex and that their rapid turnover facilitates efficient microtubule transport. Here, we discuss our findings in the larger context of cellular morphogenesis with specific focus on self-organizing principles from which cellular shape patterns such as the thin protrusions of neurons can emerge.