cDNA sequence and overexpression of chloroplast chaperonin 21 from Arabidopsis thaliana
cDNA sequence and overexpression of chloroplast chaperonin 21 from Arabidopsis thaliana
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DOI：
10.1016/s0167-4838(98)00268-4
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发表时间：
1999-01-11
期刊：
影响因子：
--
通讯作者：
Nakai, M
中科院分区：
文献类型：
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作者：
Hirohashi, T;Nishio, K;Nakai, M
高等植物叶绿体含有分子量为21 kDa的蛋白质伴侣蛋白21（Cpn 21），它是伴侣蛋白10（Cpn 10）的功能同源物。叶绿体Cpn 21多肽由串联融合在一起的两个Cpn 10样结构域组成。我们在这里描述的cDNA序列的Cpn 21（AtCpn 21）前体蛋白从拟南芥。AtCpn 21前体蛋白的推导的氨基酸序列，253个氨基酸长，显示61%的同一性与菠菜Cpn 21蛋白。AtCpn 21前体蛋白在其氨基端含有典型的叶绿体转运肽（51个氨基酸）和两个Cpn 10样结构域，它们彼此具有46%的序列同一性。AtCpn 21的预测成熟大小的多肽在大肠杆菌中表达为可溶性21-kDa蛋白。凝胶过滤和化学交联分析表明，重组成熟AtCpn 21蛋白形成一个稳定的同源寡聚体组成的三个或四个多肽。(C)1999 Elsevier Science B. V.保留所有权利。
Higher plant chloroplasts contain a 21-kDa protein, chaperonin 21 (Cpn21), that is a functional homolog of the chaperonin 10 (Cpn10). The chloroplast Cpn21 polypeptide consists of two Cpn10-like domains fused together in tandem. We describe here the cDNA sequence of the Cpn21 (AtCpn21) precursor protein from Arabidopsis thaliana. The deduced amino acid sequence of the AtCpn21 precursor protein, 253 amino acids long, shows 61% identity with the spinach Cpn21 protein. The AtCpn21 precursor protein contains the typical chloroplast transit peptide of 51 amino acids at its amino-terminus and the two Cpn10-like domains which exhibits 46% sequence identity to each other. The predicted mature-sized polypeptide of AtCpn21 was expressed in Escherichia coli as a soluble 21-kDa protein. Gel-filtration and chemical crosslinking analyses showed that the recombinant mature AtCpn21 protein forms a stable homo-oligomer composed of three or four polypeptides. (C) 1999 Elsevier Science B.V. All rights reserved.