Functional insulin-like factor 3 (INSL3) hormone-receptor system in the testes and spermatozoa of domestic ruminants and its potential as a predictor of sire fertility.
Functional insulin-like factor 3 (INSL3) hormone-receptor system in the testes and spermatozoa of domestic ruminants and its potential as a predictor of sire fertility.
复制标题
家养反刍动物睾丸和精子中的功能性胰岛素样因子 3 (INSL3) 激素受体系统及其作为雄性生育力预测因子的潜力。
DOI：
10.1111/asj.12694
复制
发表时间：
2017
影响因子：
2
通讯作者：
Kohsaka T.
中科院分区：
文献类型：
--
作者：
Pitia AM;Uchiyama K;Sano H;Kinukawa M;Minato Y;Sasada H;Kohsaka T.
胰岛素样因子 3 (INSL3) 对于胎儿睾丸下降至关重要，并且与成年男性的睾丸和精子功能有关；然而，家养反刍动物的类似功能仍然鲜为人知。本研究调查了成年反刍动物睾丸和精子中的功能性 INSL3 激素受体系统，并探讨了其诊断公牛生育力的潜力。睾丸和精子取自可育的公牛、公羊和公山羊，而低生育力的睾丸和精子仅取自公牛。正如预期的那样，INSL3 在 Leydig 细胞中可见，同时我们清楚地证明了在公牛、公羊和公山羊的睾丸生殖细胞以及精子赤道段中鉴定出了使 INSL3 能够结合的功能性受体松弛素家族肽受体 2 (RXFP2)。与可育公牛相比，可育公牛睾丸中 INSL3 和 RXFP2 表达细胞的百分比及其每个细胞的表达水平显着降低。此外，在生育力低下的公牛精液中，INSL3结合精子的数量也显着减少。这些结果为反刍动物睾丸和精子中发挥功能的 INSL3 激素受体系统及其预测公牛生育力低下的潜力提供了证据。
Insulin‐like factor 3 (INSL3) is essential for fetal testis descent, and has been implicated in the testicular and sperm functions in adult males; however, similar functions in domestic ruminants remain largely unknown. This study investigated the functional INSL3 hormone‐receptor system in adult ruminant testes and spermatozoa, and explored its potential to diagnose the fertility of sires. Testes and spermatozoa were obtained from fertile bulls, rams and he‐goats, whereas subfertile testes and spermatozoa were obtained only from bulls. As expected, INSL3 was visualized in Leydig cells, while we clearly demonstrated that the functional receptor, relaxin family peptide receptor 2 (RXFP2), enabling INSL3 to bind was identified in testicular germ cells and in the sperm equatorial segment of bulls, rams and he‐goats. In comparison to fertile bulls, the percentage of INSL3‐ and RXFP2‐expressing cells and their expression levels per cell were significantly reduced in the testes of subfertile bulls. In addition, the population of INSL3‐binding spermatozoa was also significantly reduced in the semen of subfertile bulls. These results provide evidence for a functional INSL3 hormone‐receptor system operating in ruminant testes and spermatozoa, and its potential to predict subfertility in sires.