SCREENING THE EFFECT OF NUTRITIONAL PARAMETERS ON BIOMASS AND LACCASE PRODUCTION IN SUBMERGED MEDIUM BY LITTER DECOMPOSING BASIDIOMYCETE AGARICUS HETEROCYSTIS Research Article
SCREENING THE EFFECT OF NUTRITIONAL PARAMETERS ON BIOMASS AND LACCASE PRODUCTION IN SUBMERGED MEDIUM BY LITTER DECOMPOSING BASIDIOMYCETE AGARICUS HETEROCYSTIS Research Article
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通过凋落物分解担子菌伞菌异囊筛选营养参数对沉水培养基中生物量和漆酶生产的影响研究文章
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发表时间：
2012
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影响因子：
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通讯作者：
V. Kaviyarasan
中科院分区：
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作者：
M. Manimozhi;V. Kaviyarasan
研究了印度金奈理工学院（IIT Chennai）分离的一株可食用异囊蘑菇（Agaricus heterocystis）分解凋落物在沉水培养基中的真菌生物量和漆酶产量。对培养基组成、温度、pH、碳源和氮源、金属离子等营养参数进行了优化。真菌生物量需要量采用菌丝干重法测定。结果表明，最适培养基为麦芽葡萄糖肉汤和马铃薯葡萄糖肉汤。真菌菌丝生物量产量的最佳温度为30℃，漆酶活性的最佳温度为25℃。真菌菌丝生物量产量和漆酶活性的最适pH分别为6.5和5.5。在5种碳源中，果糖支持真菌菌丝生物量最高，为175±1.50 mg/30 cm3，漆酶产量最高，为48.17±1.85 U/ml （p≤0.05）。酒石酸铵和酵母浸膏对真菌菌丝生物量产量和漆酶活性的影响最大，分别为56.89±2.52 U/ml和197±3.45 mg/30 cm3。在不同的金属离子中，只有硫酸铜具有最高的漆酶活性（50.12±3.14 U/ml）和最大的生物量（192±2.7 mg/30cm3）。讨论了这些发现的意义。
An indigenous litter decomposing edible Agaricus heterocystis isolated from IIT Chennai, India was assessed for fungal biomass and laccase production in submerged medium. Nutritional parameters such as medium composition, temperatures, pH, carbon sources and nitrogen sources, and metal ions were optimized. Fungal biomass requirements were determined by the mycelia dry weight method. The optimal medium for laccase activity and biomass production were reported as malt dextrose broth and potato dextrose broth, respectively. The optimal temperature for fungus mycelial biomass yield was recorded as 30°C, whereas the optimal temperature for laccase activity was 25°C. The optimal pH for fungi mycelial biomass yield and laccase activity were 6.5 and 5.5, respectively. Of the five carbon sources tested, fructose supported the highest fungus mycelial biomass yield of 175 ± 1.50 mg/30 cm3, and the highest laccase enzyme production with 48.17 ± 1.85 U/ml of laccase (p ≤ 0.05). The most utilized nitrogen sources for fungus mycelial biomass yield and laccase activity were ammonium tartarate and yeast extract, which stimulated the laccase production of 56.89 ± 2.52 U/ml and fungus mycelial biomass yield 197 ± 3.45 mg/30 cm3. Among the different metal ions tested, only copper sulphate showed highest laccase activity (50.12±3.14 U/ml) and maximum biomass production (192±2.7 mg/30cm3). The implications of these findings were discussed.