Effective production and purification of the glycosylated TSOL18 antigen, which is protective against pig cysticercosis
Effective production and purification of the glycosylated TSOL18 antigen, which is protective against pig cysticercosis
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DOI：
10.1128/iai.00444-07
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发表时间：
2008-02-01
影响因子：
3.1
通讯作者：
Lu, Chengping
中科院分区：
文献类型：
--
作者：
Cai, Xuepeng;Yuan, Gailing;Lu, Chengping
猪带绦虫后囊蚴病是一个世界性的公共卫生问题。在研制防治这一疾病的有效和实用疫苗方面取得了重要进展。在本研究中，有前途的T.命名为TSOL 18抗原的六钩蚴疫苗候选物在5升发酵罐中产生。在发酵过程中，培养物的pH始终保持在5.0以下，并且为了防止起泡，添加消泡剂。此外，在我们的实验中，培养物的氧含量恒定地保持在>50%。获得了高水平的糖基化蛋白（2.5g/升），并且该蛋白易于通过凝胶色谱法纯化。免疫试验表明，重组TSOL 18抗原在免疫猪中诱导了94%和100%的后绦虫负荷降低，明显高于试验1中用囊尾蚴粗提物免疫猪的89%的降低。这些是在亚洲开发控制囊虫病的有效工具方面非常有希望的结果。
Cysticercosis caused by Taenia solium metacestodes is a worldwide public health problem. Important progress in the development of effective and practical vaccines against this disease has been made. In this study, the promising T. solium oncospheral vaccine candidate named TSOL18 antigen was produced in a 5-liter fermentor. During the process of fermentation, the pH of the culture was always kept below 5.0, and in order to prevent foaming, an antifoam agent was added. In addition, the oxygen content of the culture was constantly kept at >50% in our experiment. A high level of the glycosylated protein (2.5 g/liter) was obtained, and the protein was easily purified by gel chromatography. Vaccination trials showed that the recombinant TSOL18 antigen induced 94 and 100% reductions in metacestode burdens in vaccinated pigs, obviously higher than the 89% reduction in pigs immunized with cysticercus crude extracts in trial 1. These are very promising results in the development of an efficient tool to control cysticercosis in Asia.