Influence of Selenium Content in the Culture Medium on Protein Profile of Yeast Cells Candida utilis ATCC 9950.
Influence of Selenium Content in the Culture Medium on Protein Profile of Yeast Cells Candida utilis ATCC 9950.
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DOI：
10.1155/2015/659750
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发表时间：
2015
影响因子：
--
通讯作者：
Bzducha-Wróbel A
中科院分区：
文献类型：
--
作者：
Kieliszek M;Błażejak S;Bzducha-Wróbel A
Selenium is an essential trace element for human health and it has been recognized as a component of several selenoproteins with crucial biological functions. It has been identified as a component of active centers of many enzymes, as well as integral part of biologically active complexes. The aim of the study was to evaluate the protein content and amino acid profile of the protein of fodder yeast Candida utilis ATCC 9950 cultured in media control and experimental enriched selenium. Protein analysis was performed using SDS-PAGE method consisting of polyacrylamide gel electrophoresis in the presence of SDS. The highest contents of soluble protein (49,5 mg/g) were found in yeast cells after 24-hour culture conducted in control (YPD) medium. In the presence of selenium there were determined small amounts of protein content. With increasing time of yeast culture (to 72 hours) the control and experimental media were reported to reduce soluble protein content. In electropherogram proteins from control cultures was observed the presence of 10 protein fractions, but in all the experimental cultures (containing 20, 30, and 40 mg/L selenium) of 14 protein fractions. On the basis of the molecular weights of proteins, it can be concluded that they were among others: selenoprotein 15 kDa and selenoprotein 18 kDa.
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影响因子：
3.6
作者：
MIRALLES, VJ;SERRANO, R
通讯作者：
SERRANO, R
DOI：
10.1073/pnas.93.3.1006
发表时间：
1996-02-06
影响因子：
11.1
作者：
Tamura, T;Stadtman, TC
通讯作者：
Stadtman, TC
影响因子：
3.3
作者：
McDermott JR;Rosen BP;Liu Z
通讯作者：
Liu Z
影响因子：
3.7
作者：
Peyroche, Gerald;Saveanu, Cosmin;Plateau, Pierre
通讯作者：
Plateau, Pierre
影响因子：
4.1
作者：
Chassaigne, H;Chéry, CC;Rodriguez, AR
通讯作者：
Rodriguez, AR