Bioinspired Nanofibrous Glycopeptide Hydrogel Dressing for Accelerating Wound Healing: A Cytokine-Free, M2-Type Macrophage Polarization Approach
Bioinspired Nanofibrous Glycopeptide Hydrogel Dressing for Accelerating Wound Healing: A Cytokine-Free, M2-Type Macrophage Polarization Approach
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用于加速伤口愈合的仿生纳米纤维糖肽水凝胶敷料：一种无细胞因子的 M2 型巨噬细胞极化方法
DOI：
10.1002/adfm.202006454
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发表时间：
2020-10-01
影响因子：
19
通讯作者：
Wang, Weiwei
中科院分区：
文献类型：
--
作者：
Feng, Zujian;Su, Qi;Wang, Weiwei
伤口处理仍然是临床护理中的主要问题。传统的敷料和水凝胶递送药物或细胞可以驱动伤口愈合。然而，这些方法要么受到不必要的出血和组织撕裂的限制，要么受到复杂制造、高成本和药物相关副作用的限制。本文中，合理地设计了一种新型生物启发糖肽水凝胶，以模拟皮肤细胞外基质（ECM）的糖蛋白组分和纳米纤维结构，用于通过调节巨噬细胞极化而无需任何额外的治疗剂来自加速伤口愈合。糖肽水凝胶，又称GM-肽杂化水凝胶（GP（gel）），是由β-折叠Q11肽接枝葡甘露聚糖自组装而成，具有纳米纤维结构、高含水量、多孔性和自愈合特性。观察到GP（gel）通过ERK/STAT 6途径诱导甘露糖受体活化，在体外和体内显示出显著的将原代巨噬细胞极化为M2型表型的能力。GP（凝胶）在无药物、外源性细胞因子或种子细胞的全层皮肤切除模型中前所未有地加快伤口闭合速度和表皮组织再生。GP（gel）能促进修复后皮肤组织的血管生成。总的来说，这种新型的ECM模拟糖肽水凝胶为皮肤伤口提供了一种非常有效的治疗方法，并可作为再生医学中有前途的支架。
Wound management remains a major concern in clinical care. Conventional dressings and hydrogels delivering drugs or cells can drive wound healing. However, these approaches are limited either by unnecessary bleeding and tissue tearing, or sophisticated fabrication, high cost, and drug-related side effects. Herein, a novel bioinspired glycopeptide hydrogel is rationally designed to mimic the glycoprotein components and nanofibrous architecture of cutaneous extracellular matrix (ECM) for self-accelerating the wound healing by regulating macrophage polarization without any additional therapeutic agents. The glycopeptide hydrogel, termed as GM-peptide hybrid hydrogel (GP(gel)), is established by the self-assembly of beta-sheet Q11 peptide-grafted glucomannan, with nanofibrous structure, high water content, porosity, and self-healing properties. It is observed that GP(gel)displays remarkable capability of polarizing primary macrophages to M2-type phenotype in vitro and in vivo by inducing the activation of mannose receptors through ERK/STAT6 pathway. GP(gel)unprecedentedly expedites the wound closure rate and the regeneration of epidermis tissues in full-thickness skin excision models without drugs, exogenous cytokines, or seeded cells. More significantly, GP(gel)could promote angiogenesis in the repaired skin tissues. Collectively, such a novel ECM-mimicking glycopeptide hydrogel provides a highly effective treatment approach for skin wounds and may serve as a promising scaffold in regenerative medicine.