Physiological and Molecular Mechanism of Nitric Oxide (NO) Involved in Bermudagrass Response to Cold Stress.
Physiological and Molecular Mechanism of Nitric Oxide (NO) Involved in Bermudagrass Response to Cold Stress.
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一氧化氮（NO）参与狗牙根冷应激反应的生理和分子机制
DOI：
10.1371/journal.pone.0132991
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Fu J
中科院分区：
文献类型：
--
作者：
Fan J;Chen K;Amombo E;Hu Z;Chen L;Fu J
茅草被广泛用于公园、草坪和高尔夫球场。然而，寒冷是限制狗牙根资源利用的关键因素。因此，研究狗牙根对冷胁迫的反应机制具有重要意义。一氧化氮（NO）是一种重要的信号分子，具有多种生物学功能。因此，本研究的目的是调查是否NO在狗牙根冷反应中发挥作用。以硝普钠（SNP）为NO供体，以2-苯基-4，4，5，5-四甲基咪唑啉-1-氧基-3-氧化物（PTIO）和NG-硝基-L-精氨酸甲酯（L-NAME）为NO抑制剂。将野生狗牙根在生长室中在不同处理（对照、SNP、PTIO + L-NAME）下经受4 °C。结果表明，与PTIO + L-NAME处理相比，SNP处理降低了低温胁迫下丙二醛（MDA）含量和电解质渗漏率（EL），提高了叶绿素含量、超氧化物歧化酶（SOD）和过氧化物酶（POD）活性。叶绿素（Chl a）荧光瞬变分析显示，低温胁迫下SNP处理的OJIP瞬变曲线高于PTIO + L-NAME处理。低温胁迫下，SNP处理的光合荧光参数值高于PTIO + L-NAME处理。SNP或PTIO加L-NAME处理后，冷胁迫下冷应答基因的表达发生了变化。综上所述，NO作为狗牙根抵御低温胁迫的一种重要措施，可以维持细胞膜的稳定性，上调抗氧化酶活性，恢复光系统II（PSII）的正常运转，诱导冷胁迫相关基因的表达。
Bermudagrass is widely utilized in parks, lawns, and golf courses. However, cold is a key factor limiting resource use in bermudagrass. Therefore, it is meaningful to study the mechanism of bermudagrass response to cold. Nitric oxide (NO) is a crucial signal molecule with multiple biological functions. Thus, the objective of this study was to investigate whether NO play roles in bermudagrass response to cold. Sodium nitroprusside (SNP) was used as NO donor, while 2-phenyl-4,4,5,5-tetramentylimidazoline-l-oxyl-3-xide (PTIO) plus NG-nitro-L-arginine methyl ester (L-NAME) were applied as NO inhibitor. Wild bermudagrass was subjected to 4 °C in a growth chamber under different treatments (Control, SNP, PTIO + L-NAME). The results indicated lower levels of malondialdehyde (MDA) content and electrolyte leakage (EL), higher value for chlorophyll content, superoxide dismutase (SOD) and peroxidase (POD) activities after SNP treatment than that of PTIO plus L-NAME treatments under cold stress. Analysis of Chlorophyll (Chl) a fluorescence transient displayed that the OJIP transient curve was higher after treatment with SNP than that of treated with PTIO plus L-NAME under cold stress. The values of photosynthetic fluorescence parameters were higher after treatment with SNP than that of treated with PTIO plus L-NAME under cold stress. Expression of cold-responsive genes was altered under cold stress after treated with SNP or PTIO plus L-NAME. In summary, our findings indicated that, as an important strategy to protect bermudagrass against cold stress, NO could maintain the stability of cell membrane, up-regulate the antioxidant enzymes activities, recover process of photosystem II (PSII) and induce the expression of cold-responsive genes.