Deoxyribonucleic acid repair synthesis in permeable human fibroblasts exposed to ultraviolet radiation and N-acetoxy-2-(acetylamino)fluorene.
Deoxyribonucleic acid repair synthesis in permeable human fibroblasts exposed to ultraviolet radiation and N-acetoxy-2-(acetylamino)fluorene.
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暴露于紫外线辐射和 N-乙酰氧基-2-(乙酰氨基)芴的可渗透性人成纤维细胞中脱氧核糖核酸修复合成。
DOI：
10.1021/bi00588a007
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发表时间：
1979
期刊：
影响因子：
2.9
通讯作者：
M. Lieberman
中科院分区：
文献类型：
--
作者：
J. Roberts;M. Lieberman
我们使用（3 H）dCTP、BrdUTP、dATP和dGTP作为底物研究了可渗透的、融合的正常和修复缺陷的人成纤维细胞中的脱氧核糖核酸（DNA）修复合成。在这个系统中，修复合成的发生是根据以下标准判断的：（1）标记的核苷酸掺入亲本DNA中（p = 1.73 g/cm ~ 3，在碱性CsCl中）;（2）在没有损伤的情况下，掺入亲本DNA的量可以忽略不计，而在正常细胞中，暴露于紫外线（UV）或直接作用的化学致癌物N-乙酰氧基-2-甲基-N-乙酰氧基（N-acetoxy-2-）后，掺入量增加10- 20倍。（乙酰氨基）芴;和（3）在由切除修复缺陷的人二倍体成纤维细胞（猪型干皮病细胞，互补组A）制备的制剂中不存在损伤依赖性DNA合成。将反应
We have studied deoxyribonucleic acid (DNA) repair synthesis in permeable, confluent normal and repair- deficient human fibroblasts using (3H)dCTP, BrdUTP, dATP, and dGTP as substrates. In this system repair synthesis occurred as judged by the following criteria: (1) labeled nucleotides were incorporated into parental DNA (p = 1.73 g/cm3 in alkaline CsCl); (2) incorporation into parental DNA was negligible in the absence of damage and increased 10- 20-fold in normal cells following exposure to ultraviolet ra- diation (UV) or the direct-acting chemical carcinogen N- acetoxy-2-(acetylamino)fluorene; and (3) damage-dependent DNA synthesis was absent in preparations made from excision repair-deficient human diploid fibroblasts (xeroderma pig- mentosum cells, complementation group A). The reaction was