Optimization of hydrolysis conditions of Palmaria palmata to enhance R-phycoerythrin extraction
Optimization of hydrolysis conditions of Palmaria palmata to enhance R-phycoerythrin extraction
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DOI：
10.1016/j.biortech.2012.12.146
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发表时间：
2013-03-01
影响因子：
11.4
通讯作者：
Fleurence, Joel
中科院分区：
文献类型：
--
作者：
Dumay, Justine;Clement, Nathalie;Fleurence, Joel
在本研究中，应用响应面法通过酶消化优化从红海藻 Palmaria palmata 中提取 R-藻红蛋白。首先研究了消化前的几种藻类处理。然后研究了 R-藻红蛋白的提取率和纯度指数，以及水溶性部分中蛋白质和还原糖的回收率与水解时间、温度和酶/海藻比例的关系。酶消化似乎是 R-藻红蛋白提取的有效处理方法。此外，使用湿形式粗切的海藻在提取物质量和经济成本方面给出了最有趣的结果。 R-藻红蛋白提取率比未经酶处理高 62 倍，比未经优化高 16 倍。酶法优化将纯度指数提高了16倍。 (C) 2012 Elsevier Ltd. 保留所有权利。
In this study, response surface methodology was applied to optimize R-phycoerythrin extraction from the red seaweed Palmaria palmata, using enzymatic digestion. Several algal treatments prior to digestion were first investigated. The extraction yield and the purity index of R-phycoerythrin, and the recovery of proteins and reducing sugars in the water-soluble fraction were then studied in relation to the hydrolysis time, the temperature and the enzyme/seaweed ratio.Enzymatic digestion appears to be an effective treatment for R-phycoerythrin extraction. Moreover, using the seaweed roughly cut in its wet form gives the most interesting results in terms of extract quality and economic cost. The R-phycoerythrin extraction yield is 62 times greater than without enzyme treatment and 16 times greater than without optimization. Enzymatic optimization enhanced the purity index up to 16 times. (C) 2012 Elsevier Ltd. All rights reserved.