Bioactive nanofibers: Synergistic effects of nanotopography and chemical signaling on cell guidance
Bioactive nanofibers: Synergistic effects of nanotopography and chemical signaling on cell guidance
复制标题
DOI：
10.1021/nl071182z
复制
发表时间：
2007-07-01
期刊：
影响因子：
10.8
通讯作者：
Li, Song
中科院分区：
文献类型：
--
作者：
Patel, Shyam;Kurpinski, Kyle;Li, Song
可生物降解纳米纤维在组织修复方面具有巨大的潜力。然而，纳米纤维组织和固定化生物活性因子对细胞引导的联合作用尚未得到很好的了解。在这项研究中，我们通过在纳米纤维上固定细胞外基质蛋白和生长因子，构建了具有生物活性的纳米纤维支架，模拟了天然基质原纤维的物理生化特性。与随机取向的纳米纤维相比，排列的纳米纤维在伤口愈合过程中显著诱导神经突生长和增强皮肤细胞迁移。此外，固定化的生化因子（与可溶性因子一样有效）与排列的纳米纤维协同作用，促进高效的神经突生长，但对皮肤细胞迁移的影响较小。这项研究揭示了纳米形貌和化学信号在指导不同细胞行为中的相对重要性。
Biodegradable nanofibers have tremendous potential for tissue repair. However, the combined effects of nanofiber organization and immobilized bioactive factors on cell guidance are not well understood. In this study, we developed aligned and bioactive nanofibrous scaffolds by immobilizing extracellular matrix protein and growth factor onto nanofibers, which simulated the physical and biochemical properties of native matrix fibrils. The aligned nanofibers significantly induced neurite outgrowth and enhanced skin cell migration during wound healing compared to randomly oriented nanofibers. Furthermore, the immobilized biochemical factors (as efficient as soluble factors) synergized with aligned nanofibers to promote highly efficient neurite outgrowth but had less effect on skin cell migration. This study shed light on the relative importance of nanotopography and chemical signaling in the guidance of different cell behavior.