Junction adhesion molecule is a receptor for reovirus
Junction adhesion molecule is a receptor for reovirus
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DOI：
10.1016/s0092-8674(01)00231-8
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发表时间：
2001-02-09
期刊：
影响因子：
64.5
通讯作者：
Dermody, TS
中科院分区：
文献类型：
--
作者：
Barton, ES;Forrest, JC;Dermody, TS
病毒对细胞的附着在病毒嗜性和疾病中起着至关重要的作用。呼肠孤病毒1型和3型在靶向小鼠神经系统中不同类型的细胞的能力和诱导细胞凋亡的效率方面不同。病毒附着蛋白ol与未知受体的结合控制着这些表型。我们利用表达克隆技术将紧密连接蛋白JAM鉴定为呼肠孤病毒受体。JAM直接与铝结合，并允许呼肠孤病毒感染非允许细胞。JAM的连接是呼肠孤病毒诱导的核因子-KB活化和细胞凋亡所必需的。因此，呼肠孤病毒与细胞表面受体的相互作用是细胞类型特异性趋向性和病毒诱导的最终导致细胞死亡的细胞内信号事件的关键决定因素。
Virus attachment to cells plays an essential role in Viral tropism and disease. Reovirus serotypes 1 and 3 differ in the capacity to target distinct cell types in the murine nervous system and in the efficiency to induce apoptosis. The binding of viral attachment protein ol to unidentified receptors controls these phenotypes. We used expression cloning to identify junction adhesion molecule (JAM), an integral tight junction protein, as a reovirus receptor. JAM binds directly to al and permits reovirus infection of nonpermissive cells. Ligation of JAM is required for reovirus-induced activation of NF-KB and apoptosis. Thus, reovirus interaction with cell-surface receptors is a critical determinant of both cell-type specific tropism and virus-induced intracellular signaling events that culminate in cell death.