Coordinate expression of L1 and 6B4 proteoglycan/phosphacan is correlated with the migration of mesencephalic dopaminergic neurons in mice
Coordinate expression of L1 and 6B4 proteoglycan/phosphacan is correlated with the migration of mesencephalic dopaminergic neurons in mice
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DOI：
10.1016/s0165-3806(97)00220-4
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发表时间：
1998-05-15
期刊：
影响因子：
--
通讯作者：
Kawamura, K
中科院分区：
文献类型：
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作者：
Ohyama, K;Kawano, H;Kawamura, K
小鼠的中脑多巴胺能 (DA) 神经元在胚胎第 10 天至 12 天 (E10-12) 中脑室区产生。它们首先向腹侧中脑迁移，然后在中脑的基底部分横向或切向转向。通过对 E10-E15 ICR 小鼠的免疫组织化学分析，我们发现细胞粘附分子 L1 在切向纤维的中部短暂表达，这与 DA 神经元从 E11 到 E13 的横向迁移一致，当神经元沿着切向纤维向其最终目的地移动时：网状结构、黑质致密部和腹侧被盖区。虽然在 DA 神经元中未观察到 L1 表达，但它们表达硫酸软骨素蛋白聚糖、6B4 蛋白聚糖/磷酸聚糖，该蛋白已被证明在体外与 L1/Ng-CAM 结合。这些结果表明，神经元上的 6B4 蛋白多糖与纤维上的 L1 之间的异嗜性相互作用参与了小鼠中脑 DA 神经元的横向迁移。 (C) 1998 Elsevier Science B.V.
Mesencephalic dopaminergic (DA) neurons of mice are generated from embryonic day 10 to 12 (E10-12) in the ventricular zone of the mesencephalon. They first migrate toward the ventral mesencephalon, and then turn laterally, or tangentially, in the basal part of the mesencephalon. With immunohistochemical analysis of E10-E15 ICR mice, we found that cell adhesion molecule L1 was transiently expressed on the median part of tangential fibers coincident with the lateral migration of DA neurons from E11 to E13, when neurons move along the tangential fibers toward their final destinations: the reticular formation, the substantia nigra pars compact, and the ventral tegmental area. While L1 expression was not observed in DA neurons, they expressed a chondroitin sulfate proteoglycan, 6B4 proteoglycan/phosphacan, which has been shown to bind to L1/Ng-CAM in vitro. These results suggest that the heterophilic interaction between 6B4 proteoglycan on the neurons and L1 on the fibers is involved in the lateral migration of mesencephalic DA neurons in mice. (C) 1998 Elsevier Science B.V.