Sp1 transcription factor promotes TMEPAI gene expression and contributes to cell proliferation
Sp1 transcription factor promotes TMEPAI gene expression and contributes to cell proliferation
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Sp1转录因子促进TMEPAI基因表达并有助于细胞增殖
DOI：
10.1111/cpr.12292
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发表时间：
2016-12-01
影响因子：
8.5
通讯作者：
Diao, Aipo
中科院分区：
文献类型：
--
作者：
Li, Yuyin;Guo, Ailong;Diao, Aipo
据报道，mepai（跨膜前列腺雄激素诱导蛋白）在肿瘤进展过程中过表达；然而，关于调控TMEPAI基因表达的转录机制知之甚少。材料与方法本研究鉴定并鉴定了TMEPAI基因启动子，并利用MTT法和菌落形成法评价Sp1对TMEPAI诱导的A549细胞活力的影响。结果发现-298 ~ +24序列为TMEPAI的基础启动子活性。进一步分析表明，两个Sp1结合位点对于维持TMEPAI启动子的基础转录活性至关重要，染色质免疫沉淀试验证实Sp1与TMEPAI启动子直接结合。此外，Sp1上调TMEPAI蛋白表达，Sp1促进TMEPAI诱导的细胞增殖。结论-298 ~ +24序列构成了TMEPAI的基础启动子活性。Sp1促进TMEPAI表达，促进细胞增殖。
ObjectivesTMEPAI (transmembrane prostate androgen-induced protein) has been reported to be overexpressed during tumour progression; however, little is known concerning transcriptional mechanisms regulating TMEPAI gene expression.Materials and methodsIn this study, the TMEPAI gene promoter has been identified and characterized, and the effects of Sp1 on TMEPAI-induced viability of A549 cells were evaluated, using MTT and colony formation assays.ResultsWe found that the sequence between -298 and +24 consists of basal promoter activity for TMEPAI. Further analysis indicated that two Sp1-binding sites are crucial for maintaining basal transcriptional activity of the TMEPAI promoter, and chromatin immunoprecipitation assays confirmed direct binding of Sp1 to the TMEPAI promoter. In addition, Sp1 up-regulated TMEPAI protein expression, as well as Sp1 promoting TMEPAI-induced cell proliferation.ConclusionsThese results indicate that the sequence between -298 and +24 consists of the basal promoter activity for TMEPAI. Sp1 promotes TMEPAI expression and contributes to cell proliferation.