Novel norovirus recombinants and of GII.4 sub-lineages associated with outbreaks between 2006 and 2010 in Belgium
Novel norovirus recombinants and of GII.4 sub-lineages associated with outbreaks between 2006 and 2010 in Belgium
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DOI：
10.1186/1743-422x-8-310
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发表时间：
2011-06-18
期刊：
影响因子：
4.8
通讯作者：
Dierick, Katelijne
中科院分区：
文献类型：
--
作者：
Mathijs, Elisabeth;Denayer, Sarah;Dierick, Katelijne
背景：诺如病毒（NoV）是全球人类急性胃肠炎的重要原因。为了深入了解NoV暴发的流行病学模式并确定在比利时传播的NoV菌株的遗传变异，对2006年12月至2010年12月期间食源性暴发调查中感染NoV的患者的粪便样本进行了分析。在过去4年报告的所有疑似食源性疫情中，发现11.8%是由NoV引起的，报告的NoV疫情数量沿着多年增加，占30%以上。2010年所有食源性疾病的爆发。基因组II的爆发主要占主导地位，占所有爆发的90%以上。用63个NoV阳性样品对部分聚合酶（N = 45）和/或衣壳基因（N = 35）序列进行系统发育分析。对于12个样品，获得覆盖ORF 1-ORF 2连接的序列。在基因组I和II中发现了多种基因型; GII.4占优势，其次是GII.2、GII.7、GII.13、GI.4和GI.7。在研究期间，确定了GII.4新变异体2006 a、2006 b、2007、2008和2010。此外，系统发育分析鉴定了不同的重组NoV毒株，其进一步表征为基因型间的（GII.e/GII.4，2007年，GII.e/GII.3和GII.g/GII.1）和基因亚型间（GII.4 2006 b/GII.4 2007和GII.4 2010/GII.4 2010 b）。过去4年在比利时流行的NoV通过小规模突变或遗传重组显示出显着的遗传多样性。在此期间，GII.4 2006 B被GII.4 2010亚型成功取代，先前报道的流行性GII.B重组体似乎已被2009年的GII.e重组体和2010年的GII.g重组体取代。这项研究表明，新型GII.4变体和新型GII重组体的出现可能会导致NoV疫情的爆发，与2008年和2010年观察到的情况类似。在本研究中检测到的重组体中，对两种迄今未报道的菌株GII.e/GII.3和GII.g/GII.1进行了表征。监测将仍然是重要的，以监测同时流通的菌株，以适应预防和治疗策略。
Background: Noroviruses (NoVs) are an important cause of acute gastroenteritis in humans worldwide. To gain insight into the epidemiologic patterns of NoV outbreaks and to determine the genetic variation of NoVs strains circulating in Belgium, stool samples originating from patients infected with NoVs in foodborne outbreak investigations were analysed between December 2006 and December 2010.Results: NoVs were found responsible of 11.8% of all suspected foodborne outbreaks reported in the last 4 years and the number of NoV outbreaks reported increased along the years representing more than 30% of all foodborne outbreaks in 2010. Genogroup II outbreaks largely predominated and represented more than 90% of all outbreaks. Phylogenetic analyses were performed with 63 NoV-positive samples for the partial polymerase (N = 45) and/or capsid gene (N = 35) sequences. For 12 samples, sequences covering the ORF1-ORF2 junction were obtained. A variety of genotypes was found among genogroups I and II; GII.4 was predominant followed in order of importance by GII.2, GII.7, GII.13, GI.4 and GI.7. In the study period, GII.4 NoVs variants 2006a, 2006b, 2007, 2008 and 2010 were identified. Moreover, phylogenetic analyses identified different recombinant NoV strains that were further characterised as intergenotype (GII.e/GII.4 2007, GII.e/GII.3 and GII.g/GII.1) and intersub-genotype (GII.4 2006b/GII.4 2007 and GII.4 2010/GII.4 2010b) recombinants.Conclusions: NoVs circulating in the last 4 years in Belgium showed remarkable genetic diversity either by small-scale mutations or genetic recombination. In this period, GII.4 2006b was successfully displaced by the GII.4 2010 subtype, and previously reported epidemic GII.b recombinants seemed to have been superseded by GII.e recombinants in 2009 and GII.g recombinants in 2010. This study showed that the emergence of novel GII.4 variants together with novel GII recombinants could lead to an explosion in NoV outbreaks, likewise to what was observed in 2008 and 2010. Among recombinants detected in this study, two hitherto unreported strains GII.e/GII.3 and GII.g/GII.1 were characterised. Surveillance will remain important to monitor contemporaneously circulating strains in order to adapt preventive and curative strategies.