3D porous chitosan-alginate scaffolds: a new matrix for studying prostate cancer cell-lymphocyte interactions in vitro.
3D porous chitosan-alginate scaffolds: a new matrix for studying prostate cancer cell-lymphocyte interactions in vitro.
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DOI：
10.1002/adhm.201100054
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发表时间：
2012-09
影响因子：
10
通讯作者：
Zhang, Miqin
中科院分区：
文献类型：
--
作者：
Florczyk, Stephen J.;Liu, Gang;Kievit, Forrest M.;Lewis, Allison M.;Wu, Jennifer D.;Zhang, Miqin
去势抵抗性前列腺癌（CRPC）的治疗仍然是姑息治疗。免疫疗法为 CRPC 提供了一种潜在有效的疗法；然而，其在临床上的进展缓慢，部分原因是缺乏类似于体内肿瘤微环境的代表性体外肿瘤模型来研究 CRPC 细胞与免疫细胞和其他潜在疗法的相互作用。本研究评估了 3D 多孔壳聚糖-海藻酸盐 (CA) 支架在体外培养人前列腺癌 (PCa) 细胞和研究肿瘤细胞与人外周血淋巴细胞 (PBL) 相互作用的用途。 CA 支架和 Matrigel 基质样品支持培养 15 天的体外肿瘤球体形成，CA 支架支持使用稳定转染的 PCa 细胞通过共聚焦显微镜进行活细胞荧光成像 55 天。在 Matrigel 基质和 CA 支架中生长 15 天的 PCa 细胞与 PBL 在体外共培养 2 天和 6 天，并通过扫描电子显微镜 (SEM)、免疫组织化学 (IHC) 和流式细胞术进行评估。 Matrigel 基质和 CA 支架都支持 PBL 与 PCa 肿瘤的相互作用，CA 支架为后续分析提供了更强大的平台。这项研究展示了使用3D天然聚合物支架作为组织培养模型，支持对前列腺癌肿瘤细胞与免疫细胞相互作用的长期分析，为快速免疫疗法开发提供体外平台。
The treatment of castration-resistant prostate cancer (CRPC) remains palliative. Immunotherapy offers a potentially effective therapy for CRPC; however, its advancement into the clinic has been slow, in part because of the lack of representative in vitro tumor models that resemble the in vivo tumor microenvironment for studying interactions of CRPC cells with immune cells and other potential therapeutics. This study evaluates the use of 3D porous chitosan-alginate (CA) scaffolds for culturing human prostate cancer (PCa) cells and studying tumor cell interaction with human peripheral blood lymphocytes (PBLs) ex vivo. CA scaffolds and Matrigel matrix samples supported in vitro tumor spheroid formation over 15 days of culture, and CA scaffolds supported live cell fluorescence imaging with confocal microscopy using stably transfected PCa cells for 55 days. PCa cells grown in Matrigel matrix and CA scaffolds for 15 days were co-cultured with PBLs for 2 and 6 days in vitro and evaluated with scanning electron microscopy (SEM), immunohistochemistry (IHC), and flow cytometry. Both the Matrigel matrix and CA scaffolds supported interaction of PBLs with PCa tumors, with CA scaffolds providing a more robust platform for subsequent analyses. This study demonstrates the use of 3D natural polymer scaffolds as a tissue culture model for supporting long-term analysis of interaction of prostate cancer tumor cells with immune cells, providing an in vitro platform for rapid immunotherapy development.
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影响因子：
7.4
作者：
Berencsi K;Rani P;Zhang T;Gross L;Mastrangelo M;Meropol NJ;Herlyn D;Somasundaram R
通讯作者：
Somasundaram R
影响因子：
11.5
作者：
Gulley, James L.;Drake, Charles G.
通讯作者：
Drake, Charles G.
DOI：
10.1084/jem.20091279
发表时间：
2010-01-18
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Budhu S;Loike JD;Pandolfi A;Han S;Catalano G;Constantinescu A;Clynes R;Silverstein SC
通讯作者：
Silverstein SC
影响因子：
3.7
作者：
Leung, Matthew;Kievit, Forrest M.;Florczyk, Stephen J.;Veiseh, Omid;Wu, Jennifer;Park, James O.;Zhang, Miqin
通讯作者：
Zhang, Miqin
影响因子：
48
作者：
Fischbach, Claudia;Chen, Ruth;Mooney, David J.
通讯作者：
Mooney, David J.