Down-regulation and relationship with clinicopathological parameters of LncRNA UCHL 1-AS 1 in hepatocellular carcinoma tissues
Down-regulation and relationship with clinicopathological parameters of LncRNA UCHL 1-AS 1 in hepatocellular carcinoma tissues
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发表时间：
2016
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通讯作者：
Ziling Huang;Lan-shan Huang;Kang-lai Wei;S. Shen;Chunyao Li;Weijun Li;Jian-jun Li;Yi-wu;Dang;Yan-Ping Wei;Gang Chen;Zhen-bo Feng
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作者：
Ziling Huang;Lan-shan Huang;Kang-lai Wei;S. Shen;Chunyao Li;Weijun Li;Jian-jun Li;Yi-wu;Dang;Yan-Ping Wei;Gang Chen;Zhen-bo Feng
反义泛素羧基末端水解酶 L1（反义 Uchl1，UCHL1-AS1）是 Uchl1 的剪接反义长非编码 RNA（LncRNA），可能影响人类癌症中 UCHL1 蛋白水平。本研究的目的是检测LncRNA UCHL1-AS1在肝细胞癌（HCC）中的表达，揭示UCHL1-AS1水平与临床病理参数之间的相关性。在本研究中，通过RT-qPCR在72个HCC和癌旁非癌性肝组织以及5个HCC细胞系和正常肝上皮细胞系L-O2中检测到UCHL1-AS1的表达。采用卡方检验、Kaplan-Meier法和Cox比例风险模型分析UCHL1-AS1水平与HCC临床病理参数的相关性。我们发现UCHL1-AS1在HCC中的相对表达量显着低于癌旁肝组织（P<0.0001）。 UCHL1-AS1的曲线下面积（AUC）为0.787（95% CI 0.713至0.861，P<0.0001）。另一方面，与正常肝上皮细胞系L-O2相比，在三种HCC细胞系中发现UCHL1-AS1的表达较低（P<0.05）。值得注意的是，UCHL1-AS1表达水平与肝炎病史（r=-0.336，P=0.002）、门静脉癌栓（PVTT）（r=-0.302，P=0.010）和远处转移（r=-0.235，P=0.047）显着相关。总之，这些发现支持 UCHL1-AS1 在 HCC 中下调，并且可能对预测致癌进展有价值。
Antisense ubiquitin carboxyl-terminal hydrolase L1 (antisense Uchl1, UCHL1-AS1) is a spliced antisense long noncoding RNAs (LncRNA) of Uchl1 which may influence UCHL1 protein level in human cancers. The aim of this study was to detect the expression of LncRNA UCHL1-AS1 in hepatocellular carcinoma (HCC) and reveal the correlation between UCHL1-AS1 level and clinicopathological parameters. In current study, the expression of UCHL1-AS1 was detected by RT-qPCR in 72 HCC and adjacent noncancerous liver tissues, as well as in five HCC cell lines and a normal liver epithelium cell line L-O2. The correlation of UCHL1-AS1 level with clinicopathological parameters of HCC was analyzed by the Chi-square test, Kaplan-Meier method and Cox proportional hazards model. We found that the relative expression of UCHL1-AS1 in HCC was significantly lower than that in adjacent noncancerous liver tissues (P<0.0001). The area under curve (AUC) of UCHL1-AS1 was 0.787 (95% CI 0.713 to 0.861, P<0.0001). On the other hand, lower expression of UCHL1-AS1 was found in three HCC cell lines compared to the normal liver epithelial cell line L-O2 (P<0.05). Of note, UCHL1-AS1 expression level showed significant correlation with hepatitis history (r=-0.336, P=0.002), portal vein tumor thrombus (PVTT) (r=-0.302, P=0.010) and distant metastasis (r=-0.235, P=0.047). To conclude, these findings support that UCHL1-AS1 is down-regulated in HCC and might be of value for the prediction of carcinogenic progression.