LONGITUDINAL ASSESSMENT OF FELINE IMMUNODEFICIENCY VIRUS KINETICS IN PLASMA BY USE OF A QUANTITATIVE COMPETITIVE REVERSE-TRANSCRIPTASE PCR
LONGITUDINAL ASSESSMENT OF FELINE IMMUNODEFICIENCY VIRUS KINETICS IN PLASMA BY USE OF A QUANTITATIVE COMPETITIVE REVERSE-TRANSCRIPTASE PCR
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DOI：
10.1128/jvi.69.4.2328-2332.1995
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发表时间：
1995-04-01
影响因子：
5.4
通讯作者：
HOOVER, EA
中科院分区：
文献类型：
--
作者：
DIEHL, LJ;MATHIASONDUBARD, CK;HOOVER, EA
感染猫免疫缺陷病毒（FIV）的猫发展出与人类免疫缺陷病毒1型（HIV-1）感染引起的疾病综合征相似的疾病综合征，HIV-1复制已被证明与疾病阶段和进展相关。为了评估早期FIV感染中的复制动力学和疾病进展，我们开发了一种定量竞争性逆转录酶PCR来测量病毒暴露后连续时间点的血浆病毒载量。我们发现，在感染猫的急性期症状发作之前，立即出现早期病毒血症高峰。在整个感染症状期，血浆病毒水平保持较高水平，持续8至10周，然后随着临床症状消退而下降;然而，所有猫在感染后36周内均保持显著的血浆病毒滴度。这些结果表明，在感染的急性期和继发期，FIV的动力学与HIV-1的动力学相似，血浆FIV载量与疾病阶段相关。这些结果有助于进一步发展ETV模型，并增强其在发病机制、疫苗开发、以及与艾滋病相关的治疗研究。
Cats infected with feline immunodeficiency virus (FIV) develop a disease syndrome similar to that caused by human immunodeficiency virus type 1 (HIV-1) infection in humans, HIV-1 replication has been shown to correlate with the disease stage and progression, To assess replication kinetics and disease progression in early FIV infection, we developed a quantitative competitive reverse transcriptase PCR to measure the plasma virus load at serial time points after virus exposure. We found that an early peak viremia immediately preceded the onset of acute-phase symptoms in infected cats, Plasma virus levels remained high throughout the symptomatic phase of infection, which lasted for 8 to 10 weeks, and then declined as clinical symptoms resolved; however, all cats maintained significant plasma virus titers through 36 weeks postinfection. Early peak viral replication coincided with the initial precipitous decline in circulating CD4(+) T lymphocytes, These results indicate that FIV kinetics are similar to those of HIV-1 during the acute and secondary phases of infection and that the plasma FIV load correlates with the disease stage, These results serve to further develop the ETV model and to enhance its usefulness for pathogenesis, vaccine development, and therapeutic studies related to HIV.