Expression Profiles of SV40-Immortalization-Associated Genes Upregulated in Various Human Cancers
Expression Profiles of SV40-Immortalization-Associated Genes Upregulated in Various Human Cancers
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DOI：
10.1002/jcb.22063
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发表时间：
2009-03-01
影响因子：
4
通讯作者：
Kim, Jin Kyeoung
中科院分区：
文献类型：
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作者：
Jung, Hyun Min;Choi, Seong-Jun;Kim, Jin Kyeoung
永生化是人类致癌的早期和必要的步骤，与基因表达和调控的改变有关。抑制消减杂交(SSH)被成功地用于鉴定SV40永生化肺成纤维细胞中上调的永生化相关基因。我们发现了116个与不同功能相关的已知基因，其中32.8%与细胞周期或增殖相关，表明这些基因可能参与永生化。我们选择了位于非小细胞肺癌(NSCLC)过度代表性染色体上的8个已知基因。ASPM、RFC4、C3orf26、BXDC2、C15orf44、AURKA、C20orf77和RBMX在永生化细胞、癌细胞和非小细胞肺癌(NSCLC)组织中上调。我们还克隆了两个新基因(cha-V-97和cha-V-165)，它们在所研究的细胞和组织中表现出类似的上调表达模式。对这些基因的鉴定和进一步表征可能会为永生化和人类致癌提供新的参与者的见解。J.细胞。生物化学。106：703-713,2009。(C)2009年Wiley-Liss，Inc.
Immortalization is an early and essential step or human carcinogenesis which is associated with alterations in gene expression and regulation. Suppression subtractive hybridization (SSH) was successfully performed to identify immortalization-associated genes upregulated in SV40-immortalized lung fibroblasts. We identified 116 known genes which were related to diverse functions, with 32.8% relevant for cell cycle or proliferation indicating the potential involvement of these genes in immortalization. We chose eight known genes located on the overrepresented chromosomes of non-small-cell lung cancers (NSCLCs). ASPM, RFC4, C3orf26, BXDC2, C15orf44, AURKA, C20orf77, and RBMX were upregulated in immortalized cells, cancer cells, and non-small-cell lung cancer (NSCLC) tissues. We additionally cloned tow novel genes (CHA-V-97 and CHA-V-165) which showed similar upregulated expression patterns in cells and tissues examined. Identification and further characterization of these genes may provide insights of novel players for immortalization and human carcinogenesis. J. Cell. Biochem. 106: 703-713, 2009. (C) 2009 Wiley-Liss, Inc.