UBIQUITOUS, INTERSPERSED REPEATED SEQUENCES IN MAMMALIAN GENOMES
UBIQUITOUS, INTERSPERSED REPEATED SEQUENCES IN MAMMALIAN GENOMES
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DOI：
10.1073/pnas.77.3.1398
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发表时间：
1980-01-01
期刊：
影响因子：
--
通讯作者：
SCHMID, CW
中科院分区：
文献类型：
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作者：
JELINEK, WR;TOOMEY, TP;SCHMID, CW
DNA碱基序列的比较表明，300个核苷酸的散布的人类DNA序列的主要家庭，重复的双链区域的HeLa [人宫颈癌]细胞异质核RNA和特定的RNA聚合酶III在体外转录克隆的人类DNA序列都是一个密切相关的序列家族的代表。这些序列的约30个残基的区段在哺乳动物进化中高度保守，因为它也存在于中国仓鼠的散布重复DNA序列中。进一步的DNA序列比较表明，重复哺乳动物DNA序列的高度保守片段的一部分类似于在中国仓鼠的低分子量RNA中发现的与多聚（A）终止的RNA分子氢键结合的序列，以及在乳多空病毒中DNA复制起点附近形成完美反向重复的一半的序列。
DNA base sequence comparisons demonstrate that the principal family of 300-nucleotide interspersed human DNA sequences, the repetitive double-strand regions of HeLa [human cervical carcinoma] cell heterogeneous nuclear RNA and specific RNA polymerase III in vitro transcripts of cloned human DNA sequences are all representatives of a closely related family of sequences. A segment of approximately 30 residues of these sequences is highly conserved in mammalian evolution because it is also present in the interspersed repeated DNA sequences of Chinese hamsters. Further DNA sequence comparisons demonstrate that a portion of this highly conserved segment of repetitive mammalian DNA sequence is similar to a sequence found within a low MW RNA that hydrogen-bonds to poly(A)-terminated RNA molecules of Chinese hamsters and a sequence that forms half of a perfect inverted repeat near the origin of DNA replication in papovaviruses.