REGULATION OF STROMAL CELL COLLAGENASE PRODUCTION IN ADULT-RABBIT CORNEA - INVITRO STIMULATION AND INHIBITION BY EPITHELIAL-CELL PRODUCTS
REGULATION OF STROMAL CELL COLLAGENASE PRODUCTION IN ADULT-RABBIT CORNEA - INVITRO STIMULATION AND INHIBITION BY EPITHELIAL-CELL PRODUCTS
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DOI：
10.1073/pnas.77.9.5331
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发表时间：
1980-01-01
期刊：
影响因子：
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通讯作者：
JOHNSONWINT, B
中科院分区：
文献类型：
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作者：
JOHNSONWINT, B
用成年兔角膜上皮细胞培养基能刺激和抑制相同来源的基质细胞产生潜伏性胶原酶。在这个体外系统中，细胞松弛素B是上皮细胞分泌刺激物和基质细胞产生胶原酶所必需的。刺激物的最佳生产发生在低密度上皮细胞培养中。来自这些培养物的层析条件培养基包含3个刺激分子组分，表观分子量分别为19,000，54,000和0。9万美元。高密度上皮细胞培养分泌基质细胞胶原酶的抑制物，表观分子量分别为7000和19000。细胞松弛素B不是产生抑制剂所必需的。抑制性条件培养液阻断19,000道尔顿和54,000道尔顿刺激物对基质细胞的作用。上皮细胞，根据其密度的不同，可能调节被膜中的胶原降解。
Media conditioned by epithelial cells from the adult rabbit cornea were capable of stimulating and inhibiting production of latent collagenase by stromal cells from the same source. Cytochalasin B was required in this in vitro system for secretion of stimulators by epithelial cells and production of collagenase by stromal cells in response. Optimal production of stimulators occurred in low-density epithelial cell cultures. Chromatographed conditioned medium from such cultures contained 3 stimulator fractions with apparent MW of 19,000, 54,000 and .gtoreq. 90,000. High-density epithelial cell cultures secreted inhibitors of stromal cell collagenase production with apparent MW of 7000 and 19,000. Cytochalasin B was not required for production of inhibitors. Inhibitory conditioned medium blocked the effect of the 19,000-dalton and 54,000-dalton stimulator on stromal cells. Epithelial cells, depending on their density, may modulate collagen degradation in the integument.