HIV F/3' ORF ENCODES A PHOSPHORYLATED GTP-BINDING PROTEIN RESEMBLING AN ONCOGENE PRODUCT
HIV F/3' ORF ENCODES A PHOSPHORYLATED GTP-BINDING PROTEIN RESEMBLING AN ONCOGENE PRODUCT
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DOI：
10.1038/330266a0
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发表时间：
1987-11-19
期刊：
影响因子：
64.8
通讯作者：
LECOCQ, JP
中科院分区：
文献类型：
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作者：
GUY, B;KIENY, MP;LECOCQ, JP
除了逆转录病毒gag，polandenv 1，2外，HIV基因组还含有F（3 'orf）基因，该基因编码一种N-末端被肉豆蔻酰化的206个氨基酸的多肽，其功能尚不清楚3，4。我们已经在大肠杆菌中表达了F基因，并从重组痘苗病毒中表达了VVTGfHIV。在VVTGfHIV感染的哺乳动物细胞中产生的F蛋白被豆蔻酰化，并被蛋白激酶C在靠近N末端的残基磷酸化，如pp 60-src（参考文献5）。纯化的细菌F-蛋白也显示出对therasgene产物报道的GTP酶活性、自磷酸化和GTP结合活性6，7。此外，我们还发现，在CD 4+细胞系中表达F可下调CD 4（T4）抗原。这些结果表明，F在艾滋病（获得性免疫缺陷综合征）的病理生理中具有重要作用。
Apart from the retroviralgag,polandenv1,2, the HIV genome contains theF(3'orf) gene which encodes a polypeptide of 206 amino acids which is myristylated at the N-terminal and whose function is unknown3,4. We have expressed theFgene inEscherichia coliand from a recombinant vaccinia virus, VVTGfHIV. The F-protein produced in VVTGfHIV-infected mammalian cells is myristilated, and is phosphorylated by protein kinase C at a residue close to the N-terminus like pp60-src(ref. 5). Purified bacterial F-protein also shows the GTPase, auto-phosphorylation and GTP-binding activities reported for therasgene product6,7. Furthermore, we show that expression ofFin a CD4+cell line down-regulates the CD4 (T4) antigen. These results suggest thatFis important in the pathophysiology of AIDS (aquired immune deficiency syndrome).