Nucleotide sequences and further characterization of human papillomavirus DNA present in the CaSki, SiHa and HeLa cervical carcinoma cell lines
Nucleotide sequences and further characterization of human papillomavirus DNA present in the CaSki, SiHa and HeLa cervical carcinoma cell lines
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DOI：
10.1099/0022-1317-80-7-1725
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发表时间：
1999-07-01
影响因子：
3.8
通讯作者：
Meissner, JD
中科院分区：
文献类型：
--
作者：
Meissner, JD
使用重叠的批量 PCR 产物作为模板，确定了 CaSki 和 SiHa 宫颈癌细胞系中存在的人乳头瘤病毒 16 型 (HPV-16) 变体以及 HeLa 宫颈癌细胞系中存在的初级亚基因组 HPV-18 变体的完整核苷酸序列。基于 PCR 的方法还用于表征五种先前未报告的 CaSki HPV-16 基因组破坏以及所有 HeLa HPV-18 亚基因组结构共有的 5' 细胞-病毒连接。
The complete nucleotide sequences of the human papillomavirus type 16 (HPV-16) variants present in the CaSki and SiHa cervical carcinoma cell lines and the primary subgenomic HPV-18 variant present in the HeLa cervical carcinoma cell line were determined using overlapping bulk PCR products as templates. PCR-based methods were also used to characterize five previously unreported CaSki HPV-16 genomic disruptions and the 5' cellular-viral junction common to all HeLa HPV-18 subgenomic structures.