A Novel Integrated Method for Large-Scale Detection, Identification, and Quantification of Widely Targeted Metabolites: Application in the Study of Rice Metabolomics
A Novel Integrated Method for Large-Scale Detection, Identification, and Quantification of Widely Targeted Metabolites: Application in the Study of Rice Metabolomics
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一种用于大规模检测、鉴定和定量广泛目标代谢物的新型综合方法：在水稻代谢组学研究中的应用。
DOI：
10.1093/mp/sst080
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发表时间：
2013-11-01
期刊：
影响因子：
27.5
通讯作者：
Luo, Jie
中科院分区：
文献类型：
--
作者：
Chen, Wei;Gong, Liang;Luo, Jie
基于液相色谱-质谱（LC-MS）的代谢组学研究，通过从全扫描ESI MS/MS数据构建MS（2）光谱标签（MS 2 T）库，以及使用从真实标准品收集的MS/MS数据开发广泛靶向的代谢组学方法得到了促进。在这份报告中，一种新的策略，称为逐步多离子监测增强的产物离子（逐步MIM-EPI）的开发，以构建MS 2 T库，其中逐步MIM被用作调查扫描触发采集EPI。共获得了698个（几乎）非冗余代谢物的MS（2）谱，其中135个代谢物被鉴别/注释。整合从我们的MS 2 T库收集的数据和其他可用的多反应监测（MRM）信息，开发了一种广泛针对性的代谢组学方法，以量化277种代谢物，包括一些植物激素。对水稻叶片脱水反应和这些代谢产物的自然变异的研究表明，干旱胁迫下脱落酸（阿坝）与5-羟色胺衍生物、多胺结合物等代谢产物协同调控，并揭示了一些C-糖基化黄酮类化合物可作为区分水稻籼粳亚种的潜在标记。新的MS 2 T文库构建和广泛靶向的代谢组学策略可作为水稻功能基因组学研究的工具。
Liquid chromatography-mass spectrometry (LC-MS)-based metabolomics has been facilitated by the construction of MS(2) spectral tag (MS2T) library from the total scan ESI MS/MS data, and the development of widely targeted metabolomics method using MS/MS data gathered from authentic standards. In this report, a novel strategy called stepwise multiple ion monitoring-enhanced product ions (stepwise MIM-EPI) was developed to construct the MS2T library, in which stepwise MIM was used as survey scans to trigger the acquisition of EPI. A total number of 698 (almost) non-redundant metabolites with MS(2) spectra were obtained, of which 135 metabolites were identified/annotated. Integrating the data gathered from our MS2T library and other available multiple reaction monitoring (MRM) information, a widely targeted metabolomics method was developed to quantify 277 metabolites, including some phytohormones. Evaluation of the dehydration responses and natural variations of these metabolites in rice leaf not only suggested the coordinated regulation of abscisic acid (ABA) with metabolites such as serotonin derivative(s), polyamine conjugates under drought stress, but also revealed some C-glycosylated flavones as the potential markers for the discrimination of indica and japonica rice subspecies. The new MS2T library construction and widely targeted metabolomics strategy could be used as a tool for rice functional genomics.