DETECTION OF AN OXIDIZABLE FRACTION OF CYTOCHROME-OXIDASE IN INTACT RAT-BRAIN
DETECTION OF AN OXIDIZABLE FRACTION OF CYTOCHROME-OXIDASE IN INTACT RAT-BRAIN
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DOI：
10.1152/ajpcell.1987.253.3.c477
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发表时间：
1987-09-01
影响因子：
--
通讯作者：
ROSENTHAL, M
中科院分区：
文献类型：
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作者：
LAMANNA, JC;SICK, TJ;ROSENTHAL, M
采用快速扫描反射分光光度法测定了细胞色素氧化酶在常氧大鼠脑中的还原氧化状态。在大脑皮层直接刺激引起氧化代谢活性增加后，记录了完整的血液灌注大脑皮层的反射光谱。利用无血大鼠脑和大鼠耳血管床的反射光谱对完整大鼠脑的细胞色素和血红蛋白成分进行鉴定。皮层刺激引起细胞色素氧化酶氧化增加的变化，这在反射光谱中可以检测到。对细胞色素氧化酶的可氧化部分的观察表明，细胞色素氧化酶池的一部分在常氧脑中以还原状态存在。细胞色素氧化酶的减少表明，大脑的氧气输送受到微血管控制机制的限制，这可能是脑代谢生理学的功能，也可能是对氧毒性的保护。
Rapid-scanning reflectance spectrophotometry was used to evaluate the reduction-oxidation state of cytochrome oxidase in normoxic rat brain. Reflectance spectra were recorded from intact blood-perfused cerebral cortices after increased oxidative metabolic activity induced by direct cortical stimulation. Reflectance spectra taken from blood-free rat brain and from the rat ear vascular bed were used to identify cytochrome and hemoglobin components of spectra taken from intact brain. Cortical stimulation provoked shifts toward increased oxidation of cytochrome oxidase that were detectable in reflectance spectra. Observations of an oxidizable fraction of cytochrome oxidase demonstrate that a fraction of the cytochrome oxidase pool exists in a reduced state in normoxic brain. The presence of reduced cytochrome oxidase suggests that oxygen delivery to the brain is restricted by microvascular control mechanisms either as a function of brain metabolic physiology or as protection against oxygen toxicity.