Feather-degrading Bacillus species from poultry waste
Feather-degrading Bacillus species from poultry waste
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DOI：
10.1016/s0032-9592(01)00206-0
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发表时间：
2001-11-01
影响因子：
4.4
通讯作者：
Suh, HJ
中科院分区：
文献类型：
--
作者：
Kim, JM;Lim, WJ;Suh, HJ
从家禽粪便中分离出羽毛降解细菌。在这些分离株中，被鉴定为枯草芽孢杆菌、短小芽孢杆菌和蜡状芽孢杆菌的三种菌株可有效降解羽毛，并分别产生 142、96 和 109 单位的角蛋白分解活性。 B. pumilis 和 B. cereus 产生的角蛋白分解酶可以用羽毛诱导，但 B. subtilis 在各种蛋白质（如酪蛋白、羽毛和 BSA）存在下组成型产生该酶。枯草芽孢杆菌产酶的最佳条件为40℃、pH 5-9，短小芽孢杆菌产酶的最佳条件为40℃、pH 5-6，蜡状芽孢杆菌产酶的最佳条件为30℃、pH 7.0。枯草芽孢杆菌和短小芽孢杆菌在培养84小时和72小时后的最大角质分解活性分别为161和149单位/ml，蜡状芽孢杆菌在培养60小时后的最大酶活性为117单位/ml。菌株在对数生长期后期或稳定期初期表现出最大酶活性，可溶性蛋白的产生与角蛋白分解酶具有相同的趋势。 (C) 2001 Elsevier Science Ltd. 保留所有权利。
Feather-degrading bacteria were isolated from poultry waste. Among those isolates, three strains identified as Bacillus subtilis, Bacillus pumilis and Bacillus cereus degraded feathers effectively and produced 142, 96 and 109 units of keratinolytic activities, respectively. The production of keratinolytic protease by B. pumilis and B. cereus was inducible with feathers, but B. subtilis produced the enzyme constitutively in the presence of various proteins such as casein, feather and BSA. The optimal conditions for the enzyme production by B. subtilis were 40 degreesC and pH 5-9, for B. pumilis 40 degreesC and pH 5-6 and for B. cereus 30 degreesC and pH 7.0. The maximum keratinolytic activities of B. subtilis and B. pumilis were 161 and 149 units/ml after 84 and 72 h of cultivation, respectively, With B. cereus, the maximum enzyme activity was 117 units/ml after 60 h of cultivation. The strains showed maximum enzyme activities in the late logarithmic growth phase or the beginning of stationary phase, and the production of soluble protein showed the same tendency as that of keratinolytic protease. (C) 2001 Elsevier Science Ltd. All rights reserved.