Comparative study of immunizing and delayed hypersensitivity eliciting antigens of Mycobacterium leprae, M. tuberculosis, M. vaccae, and M. bovis (BCG).
Comparative study of immunizing and delayed hypersensitivity eliciting antigens of Mycobacterium leprae, M. tuberculosis, M. vaccae, and M. bovis (BCG).
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麻风分枝杆菌、结核分枝杆菌、母牛分枝杆菌和牛分枝杆菌 (BCG) 的免疫和迟发型超敏反应诱发抗原的比较研究。
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发表时间：
1987
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通讯作者：
U. Sengupta
中科院分区：
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作者：
S. Sinha;Sreevatsa;S. Gupta;U. Sengupta
多种分枝杆菌抗原与人类和动物的延迟型超敏反应（DTH）有关。本研究试图鉴定麻风分枝杆菌和结核分枝杆菌引起DTH的主要抗原部分，并通过与两种密切相关的分枝杆菌卡介苗和母牛分枝杆菌抗原的比较来评价它们的特异性。豚鼠和小鼠分别用本地或热杀麻风分枝杆菌、结核分枝杆菌、母牛分枝杆菌和卡介苗致敏。用聚丙烯酰胺平板凝胶电泳制备后3种分枝杆菌各4个组分，用于脱除DTH。利用单克隆抗体亲和层析法获得麻风分枝杆菌的3个组分（MY1、MY2和MLSE）。热杀死的麻风分枝杆菌和母牛分枝杆菌被证明是对DTH更好的增敏剂，而高压灭菌和非高压灭菌的结核分枝杆菌或卡介苗的效果没有显著差异。结核分枝杆菌16-24 kD、卡介苗20-27 kD和麻风分枝杆菌MLSE的DTH效果最好。纯化的麻风分枝杆菌分子MY1 （12 kD）和MY2 （35 kD）没有引起DTH反应。虽然结核分枝杆菌和卡介苗的所有部分都没有表现出物种特异性，但母牛分枝杆菌和MLSE的第II部分（15-24 kD）可能由物种特异性决定因素组成。
A variety of mycobacterial antigens have been associated with the delayed-type hypersensitivity (DTH) reactions in human beings and animals. An attempt has been made in the present study to identify the major antigen fractions of Mycobacterium leprae and M. tuberculosis responsible for the elicitation of DTH and to evaluate their specificity by a comparison with the antigens of two closely related mycobacteria, BCG and M. vaccae. Guinea pigs and mice were sensitized with native or heat-killed M. leprae, M. tuberculosis, M. vaccae, and BCG. Four fractions each of the latter three mycobacteria, obtained by preparatory polyacrylamide slab gel electrophoresis, were used for elicitation of DTH. Three fractions of M. leprae (MY1, MY2, and MLSE) were obtained by affinity chromatography using monoclonal antibodies. Heat-killed M. leprae and M. vaccae proved to be better sensitizers for DTH compared to their native counterparts, whereas there were no significant differences between the efficacies of autoclaved and unautoclaved M. tuberculosis or BCG. The best DTH responses were recorded with a 16-24 kD fraction of M. tuberculosis, a 20-27 kD fraction of BCG, and MLSE of M. leprae. The purified molecules of M. leprae, MY1 (12 kD) and MY2 (35 kD), did not elicit a DTH reaction. Although none of the fractions of M. tuberculosis or BCG exhibited species specificity, fraction II (15-24 kD) of M. vaccae and MLSE might be comprised of species-specific determinants.