Administration of hydrogen sulfide protects ischemia reperfusion-induced acute kidney injury by reducing the oxidative stress
Administration of hydrogen sulfide protects ischemia reperfusion-induced acute kidney injury by reducing the oxidative stress
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DOI：
10.1007/s11845-015-1328-z
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发表时间：
2016-08-01
影响因子：
2.1
通讯作者：
Ahghari, P.
中科院分区：
文献类型：
--
作者：
Azizi, F.;Seifi, B.;Ahghari, P.
肾缺血再灌注损伤（IRI）是急性肾损伤的主要原因。硫化氢 (H2S) 被认为是一种新型气体信号分子。本研究的目的是探讨 H2S 预防肾 IRI 的功效是否是通过其抗氧化作用实现的。在这项研究中，大鼠被随机分为 Sham 组、IR 组或硫氢化钠（NaHS，H2S 供体）组。为了建立肾IRI模型，双侧肾动脉被闭塞55分钟，然后松开以允许再灌注24小时。 NaHS组大鼠在缺血开始前10分钟和再灌注开始后立即腹腔注射75 μmol/kg NaHS。假手术组在不交叉夹紧肾蒂的情况下进行剖腹手术。再灌注后，收集血浆和肾组织样本进行功能、组织学和氧化应激评估。IR组血浆肌酐、血尿素氮（BUN）、肾丙二醛（MDA）浓度显着升高，肾超氧化物歧化酶（SOD）活性显着降低。 NaHS 治疗降低了血浆肌酐、BUN、肾脏 MDA 浓度的水平，并增加了肾脏中 SOD 的活性。与 IR 组相比，NaHS 改善了肾脏组织学变化。我们的数据表明，H2S 可以预防肾 IRI，其治疗作用可能是通过减少氧化应激来介导的。
Renal ischemia-reperfusion injury (IRI) is a major cause of acute kidney injury. Hydrogen sulfide (H2S) has been known as a novel gaseous signaling molecule.The aim of this study was to investigate whether the efficacy of H2S in protecting against renal IRI is through its antioxidative effect.In this study, rats were randomized into Sham, IR, or sodium hydrosulfide (NaHS, an H2S donor) groups. To establish a model of renal IRI, both renal arteries were occluded for 55 min and then declamped to allow reperfusion for 24 h. Rats in the NaHS group received intraperitoneal injections of 75 mu mol/kg NaHS 10 min before the onset of ischemia and immediately after the onset of reperfusion. Sham group underwent laparotomy without cross-clamping of renal pedicles. After reperfusion, plasma and renal tissue samples were collected for functional, histological, and oxidative stress evaluation.The IR group exhibited significant rise in plasma creatinine, blood urea nitrogen (BUN), renal malondialdehyde (MDA) concentration, and significant reduction of renal superoxide dismutase (SOD) activity. Treatment with NaHS reduced the levels of plasma creatinine, BUN, renal MDA concentration, and increased SOD activity in the kidneys. NaHS improved renal histological changes in comparison to IR group.Our data demonstrated that H2S can protect against renal IRI and that its therapeutic effects may be mediated by reducing oxidative stress.