Photo-assisted generation of versatile zwitterionic carboxybetaine-based hypercrosslinked polymers for separation science
Photo-assisted generation of versatile zwitterionic carboxybetaine-based hypercrosslinked polymers for separation science
复制标题
用于分离科学的多功能两性离子羧基甜菜碱基超交联聚合物的光辅助生成
DOI：
10.1016/j.cej.2021.133374
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发表时间：
2022-01-06
影响因子：
15.1
通讯作者：
Jiang, Zhengjin
中科院分区：
文献类型：
--
作者：
Sun, Lingjue;Xu, Dongsheng;Jiang, Zhengjin
两性离子超亲水聚合物整体材料由于其优异的生物相容性、亲水性和亲水性而显示出迷人的属性。然而，它们的商业发展受到其繁琐的制备过程和不令人满意的性能的限制。1-羧基-N-甲基-N-二（2-甲基丙烯酰氧乙基）甲铵内盐（CBMAX）是一种重要的两性离子功能单体，具有优异的亲水性和极低的蛋白质吸附。然而，迄今为止尚未报道基于羧基甜菜碱的整料。在这项研究中，提出了一种快速的光辅助生成策略的基础上的单一单体系统（CBMAX被同时用作单体和交联剂）制备通用的超交联两性离子羧基甜菜碱聚合物在毛细管中只有5分钟。它是显着快于传统的单一单体（1小时）和多单体系统（超过12小时），通过热引发聚合方法。此外，由于羧基甜菜碱的独特优势，新型整体柱上实现了82，000板/m的高柱效，丰富的静电和亲水相互作用。这些优良的特性有利于核苷酸、酚类、苯甲酸及其衍生物、脲和尿囊素等生物活性物质在该整体柱上的高效分离。值得注意的是，这种两性离子整体柱表现出良好的抗蛋白质吸附能力以及对来自hIgG胰蛋白酶消化的N-糖肽的优异富集能力。综上所述，所提出的基于单一单体CBMAX的光辅助生成策略是非常成功的，并且为两性离子超亲水聚合物整料的商业开发开辟了一个有前途的解决方案。
Zwitterionic super-hydrophilic polymeric monoliths showed fascinating attributes because of their excellent biocompatibility, hydrophilicity, and antifouling properties. However, their commercial developments are limited by their tedious preparation process and unsatisfactory performance. 1-Carboxy-N-methyl-N-di(2-methacryloyloxyethyl)methanaminium inner salt (CBMAX), an important zwitterionic functional monomer, which exhibits excellent hydrophilicity and extremely low protein adsorption from undiluted serum. However, no carboxybetaine based monolith has been reported to date. In this study, a rapid photo-assisted generation strategy based on a sole-monomer system (CBMAX being simultaneously used as monomer and crosslinker) was proposed for preparing versatile hyper-crosslinked zwitterionic carboxybetaine polymers in capillaries within only 5 min. It is significantly faster than the conventional sole-monomer (1 h) and multi-monomer system (over 12 h) via thermally-initiated polymerization approach. Moreover, a high column efficiency of 82,000 plates/m, abundant electrostatic and hydrophilic interactions were achieved on the novel monolith due to the unique advantages of carboxybetaine. These excellent features were beneficial for the high-performance separation of various bioactive compounds (including nucleotide bases, phenols, benzoic acid and its derivatives, urea and allantoin) on this monolith. It is worth noting that this zwitterionic monolith exhibited good anti-protein adsorption ability as well as excellent enrichment capacity for N-glycopeptides from hIgG tryptic digest. In summary, the proposed photo-assisted generation strategy based on sole-monomer CBMAX is very successful and opens up a promising solution for the commercial development of zwitterionic super-hydrophilic polymeric monoliths.