Pentatricopeptide Repeat Proteins with the DYW Motif Have Distinct Molecular Functions in RNA Editing and RNA Cleavage in Arabidopsis Chloroplasts
Pentatricopeptide Repeat Proteins with the DYW Motif Have Distinct Molecular Functions in RNA Editing and RNA Cleavage in Arabidopsis Chloroplasts
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DOI：
10.1105/tpc.108.064667
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发表时间：
2009-01-01
期刊：
影响因子：
11.6
通讯作者：
Shikanaia, Toshiharu
中科院分区：
文献类型：
--
作者：
Okuda, Kenji;Chateigner-Boutin, Anne-Laure;Shikanaia, Toshiharu
植物特异性DYW亚类的五肽重复蛋白已被假定参与RNA编辑的细胞器转录本。我们发现DYW蛋白质CRR22和CRR28是编辑多种质体转录物所必需的，但它们的DYW基序在体内编辑活性是不稳定的。CRR 22和CRR 28的DYW基序被CRR 2的DYW基序取代，其已被证明能够核酸内切切割，阻断了两种蛋白质的编辑活性。反过来，CRR 22和CRR 28的DYW基序都不能在功能上取代CRR 2的DYW基序。我们认为不同的DYW家族成员在RNA成熟的不同过程中具有不同的功能，包括RNA切割和RNA编辑。
The plant-specific DYW subclass of pentatricopeptide repeat proteins has been postulated to be involved in RNA editing of organelle transcripts. We discovered that the DYW proteins CHLORORESPIRATORY REDUCTION22 (CRR22) and CRR28 are required for editing of multiple plastid transcripts but that their DYW motifs are dispensable for editing activity in vivo. Replacement of the DYW motifs of CRR22 and CRR28 by that of CRR2, which has been shown to be capable of endonucleolytic cleavage, blocks the editing activity of both proteins. In return, the DYW motifs of neither CRR22 nor CRR28 can functionally replace that of CRR2. We propose that different DYW family members have acquired distinct functions in the divergent processes of RNA maturation, including RNA cleavage and RNA editing.