Genetic Analyses of Interactions among Gibberellin, Abscisic Acid, and Brassinosteroids in the Control of Flowering Time in Arabidopsis thaliana
Genetic Analyses of Interactions among Gibberellin, Abscisic Acid, and Brassinosteroids in the Control of Flowering Time in Arabidopsis thaliana
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DOI：
10.1371/journal.pone.0014012
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发表时间：
2010-11-17
期刊：
影响因子：
3.7
通讯作者：
Davis, Seth J.
中科院分区：
文献类型：
--
作者：
Domagalska, Malgorzata A.;Sarnowska, Elzbieta;Davis, Seth J.
背景：植物激素在拟南芥开花时间调控中的遗传相互作用尚未得到广泛研究。三种植物激素分别与开花计时程序有关。赤霉素（giberellins, GAs）的归纳功能是文献记载最多的。脱落酸（ABA）已被证明具有延迟开花的作用。最后，油菜素内酯（BRs）的促进作用得到了证实。据报道，在许多生理过程中，激素途径相互作用以确保适当的生物反应。方法：我们测试了GA-， ABA-和br -依赖途径之间可能的遗传相互作用，以控制向开花的过渡。为此，我们利用缺乏GAs、ABA和BRs生物合成的单突变体和双突变体来评估激素缺乏对花转变时间的影响。在每个生物合成途径中产生过表达限速酶基因的植株，并对这些品系的开花时间进行了研究。结论：功能缺失研究揭示了GAs与ABA、ABA与BRs之间的复杂关系，并提示GAs与BRs之间存在交叉调控关系。功能增益研究表明，GAs明显限制了其作用的充分性，而BRs和ABA的增加对开花时间的表型影响更为温和。我们从基因测试中得出结论，GA、ABA和BR对花诱导时间的影响只是部分协调的作用。
Background: Genetic interactions between phytohormones in the control of flowering time in Arabidopsis thaliana have not been extensively studied. Three phytohormones have been individually connected to the floral-timing program. The inductive function of gibberellins (GAs) is the most documented. Abscisic acid (ABA) has been demonstrated to delay flowering. Finally, the promotive role of brassinosteroids (BRs) has been established. It has been reported that for many physiological processes, hormone pathways interact to ensure an appropriate biological response.Methodology: We tested possible genetic interactions between GA-, ABA-, and BR-dependent pathways in the control of the transition to flowering. For this, single and double mutants deficient in the biosynthesis of GAs, ABA, and BRs were used to assess the effect of hormone deficiency on the timing of floral transition. Also, plants that over-express genes encoding rate-limiting enzymes in each biosynthetic pathway were generated and the flowering time of these lines was investigated.Conclusions: Loss-of-function studies revealed a complex relationship between GAs and ABA, and between ABA and BRs, and suggested a cross-regulatory relation between GAs to BRs. Gain-of-function studies revealed that GAs were clearly limiting in their sufficiency of action, whereas increases in BRs and ABA led to a more modest phenotypic effect on floral timing. We conclude from our genetic tests that the effects of GA, ABA, and BR on timing of floral induction are only in partially coordinated action.